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Acetazolamide is a carbonic anhydrase (CA) IX inhibitor with an IC50 of 30 nM. Acetazolamide also inhibits hCA II with an IC50 of 130 nM. In vivo, Acetazolamide (40 mg/kg) significantly potentiates the inhibitory effect of MS-275 on tumorigenesis in neuroblastoma (NB) SH-SY5Y xenografts.
| Cell Experiment | |
|---|---|
| Cell lines | renal carcinoma cell lines(Caki-1, Caki-2, ACHN, and A-498) |
| Preparation method | The invasion assay is performed in a Biocoat Matrigel invasion chamber. Cells (n = 1.8 × 105) are added to the chamber and cultured for 48 h in the presence or absence of acetazolamide, which was administered twice during the 48-h period: the first dose at the beginning of the assay and the second after 24 h. After growing for 48 h, noninvasive cells are removed from the upper surface of the membrane with a cotton swab. The invasive cells on the lower surface of the membrane are fixed with 4% (vol/vol) neutral-buffered formaldehyde, stained with toluidine blue, and counted in 20 separate areas with a Leitz Diaplan microscope. |
| Concentrations | 10 μM |
| Incubation time | 48 h |
| Animal Experiment | |
|---|---|
| Animal models | Male Sprague-Dawley rats |
| Formulation | - |
| Dosages | 10, 100, 200 mg/kg |
| Administration | i.p. |
| Molecular Weight | 222.25 |
| Formula | C4H6N4O3S2 |
| CAS Number | 59-66-5 |
| Form | Solid |
| Solubility (25°C) | DMSO ≥ 25 mg/mL |
| Storage | 2-8°C, dry, protect from light, sealed |
| Related Carbonic Anhydrase Products |
|---|
| hCAIX-IN-5
hCAIX-IN-5 is a potent and selective hCAIX inhibitor with KIs of >10000, >10000, 130.7, 829.1 nM for hCAI, hCAII, hCAIV, hCAIX, respectively. |
| Disulfamide
Disulfamide, an orally active diuretic, is a carbonic anhydrase inhibitor with the IC50 value of 0.07 μM. |
| Quinethazone
Quinethazone is an orally active diuretic agent and is also a weak inhibitor of carbonic anhydrase. |
| Acetazolamide sodium
Acetazolamide sodium is a carbonic anhydrase (CA) IX inhibitor with an IC50 of 30 nM for hCA IX.Acetazolamide reduces the production of intraocular aqueous humor and may be used in glaucoma-related studies. |
| WES-1
WES-1 is an inhibitor of carbonic anhydrase IX (Ki: 55.9 μM). |
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