7-aminoactinomycin D (7-aad) can bind to DNA, and the maximum excitation/emission wavelength is 546nm/647 nm. The best excitation laser is 561nm or 488nm. It gives red fluorescence and is often used to observe the nucleus and nucleic acid. Store in freezer (-5 to -30°C) away from light. The solvent was DMSO.
*The compound is unstable in solutions, freshly prepared is recommended
Ecotoxicol Environ Saf. 2022 Jun 1;237:113564.
Environmental BPDE induced human trophoblast cell apoptosis by up-regulating lnc-HZ01/p53 positive feedback loop
7-Aminoactinomycin D purchased from AbMole
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Source | Am J Vet Res (2015). Figure 4. 7-Aminoactinomycin D |
| Method | apoptosis assay | |
| Cell Lines | Human Jurkat T cells | |
| Concentrations | 1 μg/ml | |
| Incubation Time | 4 h | |
| Results | It is worth noting that although membrane-impermeable nucleic acid–binding dyes such as PI, 7-AAD and TO-PRO-3 alone are sufficient to monitor the generation of membrane- permeabilized cells and a procedure used by many investigators to study primary necrosis, necroptosis and pyroptosis |
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Source | Am J Vet Res (2015). Figure 3. 7-Aminoactinomycin D |
| Method | Flow cytometry analysis | |
| Cell Lines | Human Jurkat T cells | |
| Concentrations | 1 μg/ml | |
| Incubation Time | 4 h | |
| Results | To accommodate 7-AAD staining in the data analysis when all three viability stains are used simultaneously, stage 1 of the seven-stage gating strategy is replaced with an alternative stage 1, with 7-AAD plotted against A5-FITC to identify A5-FITC high and 7-AAD high particles as membrane-permeabilized cells. |
| Cell Experiment | |
|---|---|
| Cell lines | Normal human embryonic lung fibroblasts |
| Preparation method | The corresponding data were obtained for binding of 7-AAMD using dye concentrations from 0.5 to 20 pM. The dye uptake for the dye concentration 0.5 pM was completed after 3 h. Staining with 7-AAMD resulted in higher background fluorescence, especially for unextracted samples, where it started at 15% of the nuclear fluorescence for the lowest dye concentration and amounting to about 40% for the highest. |
| Concentrations | 0.5 to 20 μM |
| Incubation time | 3 h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 1270.43 |
| Formula | C62H87N13O16 |
| CAS Number | 7240-37-1 |
| Solubility (25°C) | DMSO 10 mM |
| Storage | -20°C, protect from light |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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|---|
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Cyanine 5 Tyramide (Tyramide-Cy5) methyl indole is a red fluorescent dye. |
| 2-Aminopurine
2-Aminopurine has been used to inhibit eukaryotic initiation factor-2α (eIF2α)-phosphorylation of osteoarthritis (OA) chondrocytes. 2-Aminopurine, a fluorescent analog of guanosine and adenosine, is a widely used fluorescence-decay-based probe of DNA structure. 2-Aminopurine has been used to probe nucleic acid structure and dynamics. |
| DFHBI 1T
DFHBI 1T is a mimic of GFP fluorophore for imaging RNA in living cells. DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. |
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