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7-Aminoactinomycin D

Cat. No. M4849

All AbMole products are for research use only, cannot be used for human consumption.

7-Aminoactinomycin D Structure
Synonym:

7-AAD

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1mg USD 225  USD225 In stock
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Quality Control & Documentation
Biological Activity

7-aminoactinomycin D (7-aad) can bind to DNA, and the maximum excitation/emission wavelength is 546nm/647 nm. The best excitation laser is 561nm or 488nm. It gives red fluorescence and is often used to observe the nucleus and nucleic acid. Store in freezer (-5 to -30°C) away from light. The solvent was DMSO.

*The compound is unstable in solutions, freshly prepared is recommended



Product Citations
Customer Product Validations & Biological Datas
Source Am J Vet Res (2015). Figure 4. 7-Aminoactinomycin D
Method apoptosis assay
Cell Lines Human Jurkat T cells
Concentrations 1 μg/ml
Incubation Time 4 h
Results It is worth noting that although membrane-impermeable nucleic acid–binding dyes such as PI, 7-AAD and TO-PRO-3 alone are sufficient to monitor the generation of membrane- permeabilized cells and a procedure used by many investigators to study primary necrosis, necroptosis and pyroptosis
Source Am J Vet Res (2015). Figure 3. 7-Aminoactinomycin D
Method Flow cytometry analysis
Cell Lines Human Jurkat T cells
Concentrations 1 μg/ml
Incubation Time 4 h
Results To accommodate 7-AAD staining in the data analysis when all three viability stains are used simultaneously, stage 1 of the seven-stage gating strategy is replaced with an alternative stage 1, with 7-AAD plotted against A5-FITC to identify A5-FITC high and 7-AAD high particles as membrane-permeabilized cells.
Protocol (for reference only)
Cell Experiment
Cell lines Normal human embryonic lung fibroblasts
Preparation method The corresponding data were obtained for binding of 7-AAMD using dye concentrations from 0.5 to 20 pM. The dye uptake for the dye concentration 0.5 pM was completed after 3 h. Staining with 7-AAMD resulted in higher background fluorescence, especially for unextracted samples, where it started at 15% of the nuclear fluorescence for the lowest dye concentration and amounting to about 40% for the highest.
Concentrations 0.5 to 20 μM
Incubation time 3 h
Animal Experiment
Animal models
Formulation
Dosages
Administration
Chemical Information
Molecular Weight 1270.43
Formula C62H87N13O16
CAS Number 7240-37-1
Solubility (25°C) DMSO 10 mM
Storage -20°C, protect from light
References

[1] Rundquist I, et al. Cytometry. Equilibrium binding of DAPI and 7-aminoactinomycin D to chromatin of cultured cells.

[2] Wadkins RM, et al. Biochemistry. Actinomycin D and 7-aminoactinomycin D binding to single-stranded DNA.

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Keywords: 7-Aminoactinomycin D, 7-AAD supplier, Nucleic Acid Staining, inhibitors, activators

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