2-Aminopurine, a fluorescent analog of guanosine and adenosine, is a widely used fluorescence-decay-based probe of DNA structure. 2-Aminopurine has been used to probe nucleic acid structure and dynamics. 2-Aminopurine has been used to inhibit eukaryotic initiation factor-2α (eIF2α)-phosphorylation of osteoarthritis (OA) chondrocytes. 2-Aminopurine (2AP) is an analog of guanosine and adenosine, which can base pair with cytosine and thymine. It is used as a fluorescent probe in nucleic acid structure and dynamics. 2-Aminopurine (2-AP) inhibits double-stranded RNA-dependent protein kinase, protein kinase R (PKR).
| Molecular Weight | 135.13 |
| Formula | C5H5N5 |
| CAS Number | 452-06-2 |
| Solubility (25°C) | formic acid:water (1:1 v/v) (50 mg/ml) with heating |
| Storage | 2-8°C, protect from light, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Pai Peng, et al. Analyst. DNA nanodevices monitored with fluorogenic looped-out 2-aminopurine
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| DFHBI 1T
DFHBI 1T is a mimic of GFP fluorophore for imaging RNA in living cells. DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. |
| 7-Aminoactinomycin D
7-aminoactinomycin D (7-aad) can bind to DNA, and the maximum excitation/emission wavelength is 546nm/647 nm. The best excitation laser is 561nm or 488nm. It gives red fluorescence and is often used to observe the nucleus and nucleic acid. Store in freezer (-5 to -30°C) away from light. The solvent was DMSO. *The compound is unstable in solutions, freshly prepared is recommended |
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