Thapsigargin is a potent inhibitor of sarco-endoplasmic reticulum Ca2+-ATPases. Thapsigargin causes ER stress; Thapsigargin can be used to induce autophagy in mammalian cells. Thapsigargin inhibits cell growth in a time-dependent manner, especially in the HepG2 cells, for which 64% cell growth is inhibited at 96 h compared with 45% in the HepG2.2.15 cells (P<0.001).
J Food Sci. 2023 Jan;88(1):523-536.
Effect of Flammulina velutipes polysaccharides on endoplasmic reticulum stress-mediated apoptosis by activating PLC–IP3 pathway in HepG2 cells
Thapsigargin purchased from AbMole
| Molecular Weight | 650.76 |
| Formula | C34H50O12 |
| CAS Number | 67526-95-8 |
| Solubility (25°C) | DMSO 40 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[3] Geiszt M, et al. Biochem J. Thapsigargin inhibits Ca2+ entry into human neutrophil granulocytes.
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