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Streptavidin (High efficiency)

Cat. No. M10174
Streptavidin (High efficiency) Structure
Size Price Availability Quantity
1mg USD 120  USD120 In stock
5mg USD 400  USD400 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
  • Purity: ≥17 units/mg protein
  • COA
  • MSDS
Biological Activity

Streptavidin (High efficiency) is a biotin binding protein and has the ability to bind four molecules of biotin.

Gives a clear solution at 5mg/mL in 0.1M NaCl. Streptavidin is soluble in water at 1 mg/mL, or in phosphate buffered saline (PBS).

Chemical Information
Molecular Weight 378.71
Formula C14H17BrClNO2S
CAS Number 9013-20-1
Solubility (25°C) Water 1 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Yalong Cong, et al. Nanoscale. Entropic effect and residue specific entropic contribution to the cooperativity in streptavidin-biotin binding

[2] Clemens Wolfesberger, et al. J Nanobiotechnology. Streptavidin functionalized polymer nanodots fabricated by visible light lithography

[3] Steven K Taylor, et al. Angew Chem Int Ed Engl. Monovalent streptavidin that senses oligonucleotides

[4] Kok Hong Lim, et al. Biotechnol Bioeng. Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection

[5] Claire E Chivers, et al. Nat Methods. A streptavidin variant with slower biotin dissociation and increased mechanostability

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