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Recombinant Human SCF (P. pastoris)

Cat. No. M11493

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human SCF (P. pastoris) Structure
Synonym:

Kit ligand; C-Kit Ligand; Mast Cell Growth Factor

Size Price Availability Quantity
10ug USD 190  USD190 In stock
50ug USD 310  USD310 In stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin < 1 EU/μg
  • COA
  • MSDS
Biological Activity

Expression system: P. pastoris
Purity: SDS-PAGE > 95%
Endotoxin level: protein < 1 EU/μg was determined by gel coagulation
Apparent molecular weight: ~ 20kDa, reduced by SDS-PAGE
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.


Biological activity: A stem cell factor  (P. pastoris) (also known as SCF, KIT ligand, KL, or steel factor) is a cytokine that binds to the C-kit receptor (CD117). SCF can exist as both transmembrane protein and soluble protein. In bone marrow culture, it stimulates the proliferation of myeloid, red blood cell and lymphoid progenitor cells and has been shown to act synergistically with colony-stimulating factors. SCF plays an important role in the hematopoietic process of embryonic development. SCF can regulate hematopoietic stem cells in the niche of bone marrow stem cells. SCF has been shown to increase the survival rate of hematopoietic stem cells in vitro and contribute to self-renewal and maintenance of hematopoietic stem cells in vivo.

Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Laura L Thompson, et al. Int J Mol Sci. The SCF Complex Is Essential to Maintain Genome and Chromosome Stability

[2] Gitana Mickiene, et al. PeerJ. Human granulocyte-colony stimulating factor (G-CSF)/stem cell factor (SCF) fusion proteins: design, characterization and activity

[3] Zeyuan Cao, et al. Stem Cell Res Ther. Hepatocyte growth factor (HGF) and stem cell factor (SCF) maintained the stemness of human bone marrow mesenchymal stem cells (hBMSCs) during long-term expansion by preserving mitochondrial function via the PI3K/AKT, ERK1/2, and STAT3 signaling pathways

[4] Danrui Cui, et al. Cell Rep. FBXW7 Confers Radiation Survival by Targeting p53 for Degradation

[5] Moein Dehbashi, et al. Mol Biol Res Commun. Comparative genomics of human stem cell factor (SCF)

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Keywords: Recombinant Human SCF (P. pastoris), Kit ligand; C-Kit Ligand; Mast Cell Growth Factor supplier, Cytokines and Growth Factors, inhibitors, activators

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