Expression system: P. pastoris
Purity: SDS-PAGE > 95%
Endotoxin level: protein < 1 EU/μg was determined by gel coagulation
Apparent molecular weight: ~ 20kDa, reduced by SDS-PAGE
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Biological activity: A stem cell factor (P. pastoris) (also known as SCF, KIT ligand, KL, or steel factor) is a cytokine that binds to the C-kit receptor (CD117). SCF can exist as both transmembrane protein and soluble protein. In bone marrow culture, it stimulates the proliferation of myeloid, red blood cell and lymphoid progenitor cells and has been shown to act synergistically with colony-stimulating factors. SCF plays an important role in the hematopoietic process of embryonic development. SCF can regulate hematopoietic stem cells in the niche of bone marrow stem cells. SCF has been shown to increase the survival rate of hematopoietic stem cells in vitro and contribute to self-renewal and maintenance of hematopoietic stem cells in vivo.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] Danrui Cui, et al. Cell Rep. FBXW7 Confers Radiation Survival by Targeting p53 for Degradation
[5] Moein Dehbashi, et al. Mol Biol Res Commun. Comparative genomics of human stem cell factor (SCF)
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