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RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity.
| Cell Experiment | |
|---|---|
| Cell lines | PC3 cells |
| Preparation method | PC3 cells were treated with 60 μM RS-1 for the indicated times. Cells were collected and fixed in ice cold 70% ethanol for at least 4 hours. Cells were rinsed and stained with 50 μg/ml PI, 50 μg/ml RNAse A in PBS for 20 minutes. Cell cycle distributions were analyzed by flow cytometry using a LSR-II (BD biosciences) cytometer. The percentage of cells in G1, S, and G2/M phases of the cell cycle was determined using FlowJo software. |
| Concentrations | 60 μM |
| Incubation time | 24 h |
| Animal Experiment | |
|---|---|
| Animal models | Athymic nude mice |
| Formulation | 30% DMSO, 35% PEG-400, 35% PBS |
| Dosages | 200 μl |
| Administration | Peritoneal administrations |
| Molecular Weight | 524.23 |
| Formula | C20H16Br2N2O3S |
| CAS Number | 312756-74-4 |
| Solubility (25°C) | DMSO 60 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[1] Song J, et al. Nat Commun. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency.
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| SCR7 pyrazine
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