Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm). Rhod-2 AM is cell-permeable version of Rhod-2. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers.
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
|Purity||>98%, Biological Stain|
|Storage||-20°C, protect from light, dry, sealed|
|Related Fluorescent Dye Products|
Diphenylterazine is a bioluminescence agent. Diphenylterazine alone yields very little background, leading to excellent signal-to-background ratios. DMSO can inactivate Diphenylterazine's activity
X-Gluc Dicyclohexylamine is used as a reagent to detect β-glucuronidase, an enzyme produced by the E. Coli bacterium; is widely used in molecular biology experiments to mark and select the expression of target genes (GUS reporter system).
|JC-1 Mitochondrial Membrane Potential Assay Kit
JC-1 Mitochondrial Membrane Potential Assay Kit is a kit that uses JC-1 as a fluorescent probe to quickly and sensitively detect the transmembrane potential difference of tissues, cells or purified mitochondria. It can be used for early apoptosis detection.
Ruthenium red (Ammoniated ruthenium oxychloride) is a polycationic dye widely used for electron microscopy (EM) of cells, tissues and vegetative bacteria. Ruthenium red is also a L-type calcium current (ICa) blocker, blocks Ca2+ uptake and release, and voltage-sensitive Ca2+ channels.
|Calcein Red, AM
Calcein AM is one of the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. Non-fluorescent Calcein Red AM can easily get into live cells and hydrolyzes to generate strongly fluorescent Calcein Red dye. Calcein Red dye can be monitored with the common TRITC/Cy3 filter set.
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