Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm). Rhod-2 AM is cell-permeable version of Rhod-2. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers.
Molecular Weight | 1123.96 |
Formula | C52H59BrN4O |
CAS Number | 145037-81-6 |
Solubility (25°C) | DMSO 1~5mM |
Storage | -20°C, protect from light, dry, sealed |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
Related Fluorescent Dye Products |
---|
FM1-43
FM1-43 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers. |
BCIP
BCIP is a chromogenic substrate for colorimetric assay of alkaline phosphatase activity. |
MCA-SEVNLDAEFR-K(Dnp)-RR, amide
MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate. |
MeOSuc-AAPV-AMC
MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm). |
Enhanced Green Fluorescent Protein (EGFP) (200-208)
Enhanced Green Fluorescent Protein (EGFP) (200-208) is a marker gene product derived from the jellyfish Aequorea Victoria. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.