Species: Mouse
Expression system: E.coli
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: less than 0.001 ng/ug (0.01 EU/ug) as determined by LAL
Storage and stability: Lyophilized proteins should be stored at < -20°C but can be stored at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.
Biological Activity: Mouse Sonic Hedgehog Homolog (SHH) belongs to the Hedgehog triprotein family. The other two family members are the Indian hedgehog (IHH) and desert hedgehog (DHH). Hedgehog proteins are key signaling molecules for embryonic development. SHH is expressed in a variety of embryonic tissues and plays a key role in regulating the patterns of many systems, from limbs to the brain. SHH also plays an important role in adults, including the division of adult stem cells and the development of certain cancers and other diseases. Mouse Shh is synthesized as a 437AA precursor containing a 24AA signal sequence and a 413AA maturation region. The mature region was autocatalytically processed into a non-glycosylated, 20kDa, 174 AA N-terminal fragment (SH-N) and a catalytically processed, 34 kDa, 239 AA C-terminal fragment. Shh-n fragment of 20 kDa is the core of active hedgehog. The homology of mouse SHH-N with human SHH-N, rat SHH-N and gerbil SHH-N was 99%, 98% and 100%, respectively.
| Solubility (25°C) | It is not recommended to reconstitute to a concentration less than 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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