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Recombinant Human TIMP-2 (HEK293,C-6His)

Cat. No. M15012

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human TIMP-2 (HEK293,C-6His) Structure
Synonym:

Metalloproteinase Inhibitor 2; CSC-21K

Size Price Availability Quantity
10ug USD 280  USD280 In stock
50ug USD 840  USD840 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin <1 EU/ug
  • COA
  • MSDS
Biological Activity

Tissue metalloproteinase inhibitor 2 (TIMP-2) is a non-N-glycosylated protein with a molecular weight of 22 kDa. It is produced by a variety of cell types and inhibits matrix metalloproteinases in a non-covalent manner by forming binary complexes and irreversibly inactivates matrix metalloproteinases by binding to zinc cofactors. Timp-2 also has the activity of promoting erythrocyte growth and cell growth.

Recombinant Human Tissue Inhibitor of Metalloproteinases 2 expressed the target gene encoding Cys27-Pro220 with a 6His tag at the C-terminus.


Accession: P16035

Apparent Molecular Weight: 26 KDa, reducing conditions

Endotoxin < 1 EU/µg

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in distilled water to a concentration not less than 100 μg/mL.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
References

[1] Aiying Xue, et al. Sheng Wu Gong Cheng Xue Bao. [Prokaryotic expression, purification and characterization of tissue inhibitor of metalloproteinase-2]

[2] Lena Vinther, et al. Protein Expr Purif. Purification and characterization of bioactive his6-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells

[3] L Xu, et al. Gut. Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis

[4] Heidi S-T Kai, et al. J Biol Chem. Utilization of a novel recombinant myoglobin fusion protein expression system to characterize the tissue inhibitor of metalloproteinase (TIMP)-4 and TIMP-2 C-terminal domain and tails by mutagenesis. The importance of acidic residues in binding the MMP-2 䲧璵Ỵ璶㧀璴羹癦

[5] R Fridman, et al. Biochem J. Expression of human recombinant 72 kDa gelatinase and tissue inhibitor of metalloproteinase-2 (TIMP-2): characterization of complex and free enzyme

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Keywords: Recombinant Human TIMP-2 (HEK293,C-6His), Metalloproteinase Inhibitor 2; CSC-21K supplier, Cytokines and Growth Factors, inhibitors, activators

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