Sonic Hedgehog Homolog (SHH) belongs to the Hedgehog family of three proteins. The other two family members are the Indian hedgehog (IHH) and desert hedgehog (DHH). Hedgehog proteins are key signaling molecules for embryonic development. SHH is expressed in a variety of embryonic tissues and plays a key role in regulating the patterns of many systems, from limbs to the brain. SHH also plays an important role in adults, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor and undergoes a series of processes during secretion. After the removal of the signal peptide, a protease located in the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). Shh-n has "full signal" capability. Shh-n binds to Patched 12-channel transmembrane proteins (Ptc) on the cell surface to release Ptc's inhibitory effect on THE activity of G protein-coupled receptor Smoothened (Smo).
Recombinant Human Sonic Hedgehog expressed the target gene encoding Cys24-Gly197.
Accession: Q15465
Endotoxin < 0.01 EU/µg
Apparent Molecular Weight: 19 KDa, reducing conditions
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Haiying Zhou, et al. Mol Cell Biol. Mediator modulates Gli3-dependent Sonic hedgehog signaling
[2] V Marigo, et al. Nature. Biochemical evidence that patched is the Hedgehog receptor
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