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Recombinant Human NT-proBNP ( E.coli, N-6His-Flag)

Cat. No. M21348

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human NT-proBNP ( E.coli, N-6His-Flag) Structure
Synonym:

Natriuretic peptides B; Gamma-brain natriuretic peptide

Size Price Availability Quantity
10ug USD 200  USD200 In stock
50ug USD 505  USD505 In stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

Genus: Human
Expression system: E.coli
Purity: >95% by reduced SDS-PAGE
Molecular weight: 11 KDa
Endotoxin: <0.1 ng/µg (1 EU/µg) by LAL test
Storage and Stability: Lyophilized proteins should be stored at <-20°C, although stable at room temperature for up to 3 weeks. Reconstituted protein solutions can be stored at 4-7°C for 2-7 days. Recombinant sample aliquots are stable for 3 months at <-20°C.


Bioactivity: Brain-type natriuretic peptide (BNP) is a non-glycosylated peptide produced mainly by ventricular myocytes and belongs to the natriuretic peptide family. 12 kDa of BNP precursor is proteolytically cleaved to produce N-terminal pro-BNP (NT-proBNP) and mature BNP. N-terminal pro-B-type natriuretic peptide (NT-proBNP) is a useful marker of heart failure (HF). Thought to be secreted primarily from the ventricles, increases in serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defects in patients without HF.

Chemical Information
Solubility (25°C) It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Zhihua Pang, et al. Medicine (Baltimore). A study of the sequential treatment of acute heart failure with sacubitril/valsartan by recombinant human brain natriuretic peptide: A randomized controlled trial

[2] Marc Humbert, et al. N Engl J Med. Sotatercept for the Treatment of Pulmonary Arterial Hypertension

[3] Amir Tajbakhsh, et al. Expert Rev Anti Infect Ther. COVID-19 and cardiac injury: clinical manifestations, biomarkers, mechanisms, diagnosis, treatment, and follow up

[4] Tomasz J Guzik, et al. Cardiovasc Res. COVID-19 and the cardiovascular system: implications for risk assessment, diagnosis, and treatment options

[5] Muhammad Tarmizi Soleh, et al. Biotechnol Lett. A rapid and cost-effective method of producing recombinant proBNP and NT-proBNP variants in Escherichia coli for immunoassay of heart failure

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Keywords: Recombinant Human NT-proBNP ( E.coli, N-6His-Flag), Natriuretic peptides B; Gamma-brain natriuretic peptide supplier, Cytokines and Growth Factors, inhibitors, activators

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