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Recombinant Human MIP-1β /CCL4 (E. coli)

Cat. No. M15026

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human MIP-1β /CCL4 (E. coli) Structure
Synonym:

MIP-1 beta; Macrophage Inflammatory Protein-1β; CCL-4; ACT-2

Size Price Availability
5ug USD 165  USD165 Out of stock
25ug USD 280  USD280 Out of stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Species: Human
Expression system: E. coli
Purity: SDS-PAGE analysis, > 95%, HIGH performance liquid chromatography analysis, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~7.6 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles


Bioactivity: The EC50 value of human MIP-1 β /CCL4 against Cho-K1 / Gα15/hCCR5 cells (in which human Gα15 and human CCR5 were stably expressed) was less than 100.0 ng/ mL. Mip-1 β is a major HIV suppressor produced by CD8+ T cells. Perforin-low Memory CD8+ T cells are the most common T cells that synthesize MIP-1 β in humans. Mip-1 β has been shown to interact with CCL3. It can signal through the CCR5 receptor.


Chemical Information
Solubility (25°C) It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Christy S Barrios, et al. Viral Immunol. Recombinant human T-cell leukemia virus types 1 and 2 Tax proteins induce high levels of CC-chemokines and downregulate CCR5 in human peripheral blood mononuclear cells

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Keywords: Recombinant Human MIP-1β /CCL4 (E. coli), MIP-1 beta; Macrophage Inflammatory Protein-1β; CCL-4; ACT-2 supplier, Cytokines and Growth Factors, inhibitors, activators

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