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Recombinant Human CD3E (Mammalian, C-mFc)

Cat. No. M21571
Recombinant Human CD3E (Mammalian, C-mFc) Structure
Synonym:

T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain

Size Price Availability Quantity
10ug USD 160  USD160 In stock
50ug USD 360  USD360 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin<1 EU/µg
  • COA
  • MSDS
Biological Activity

Species: Human
Expression system: Mammalian
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: LAL test less than 0.1 ng/µg (1 EU/µg)
Molecular weight: 28.5 KDa
Storage and stability: The freeze-dried protein should be stored at ≤-20℃ and stored stably for one year after receiving. The recombinant protein solution can be stored at 2-8℃ for 2-7 days. The recombinant samples were stable for 3 months at ≤-20°C.



Bioactivity: t cell surface glycoprotein CD3ε Chain is a single channel type I membrane protein. CD3ε contains one immunoglobulin-like domain and one ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on human chromosome 11. T cell receptor -CD3 complex (TCR/CD3 complex) is involved in T cell development and several intracellular signal transduction pathways. This complex is essential for t cell development and function and is one of the most complex transmembrane receptors. T cell receptor - cd3 complexes have 10 tyrosine-based cytoplasmic immune receptor activation primitives (ITAMs). The TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal transduction pathways.

Chemical Information
Solubility (25°C) It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Charlotte A James, et al. Nat Commun. CD4 and CD8 co-receptors modulate functional avidity of CD1b-restricted T cells

[2] Kamonlapat Supimon, et al. Sci Rep. Anti-mucin 1 chimeric antigen receptor T cells for adoptive T cell therapy of cholangiocarcinoma

[3] Martin Hart, et al. Cell Death Dis. miR-34a: a new player in the regulation of T cell function by modulation of NF-κB signaling

[4] Jane Rasaiyaah, et al. JCI Insight. TCRαβ/CD3 disruption enables CD3-specific antileukemic T cell immunotherapy

[5] Sinem Firtina, et al. Immunogenetics. A novel pathogenic frameshift variant of CD3E gene in two T-B+ NK+ SCID patients from Turkey

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Keywords: Recombinant Human CD3E (Mammalian, C-mFc), T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain supplier, Recombinant Proteins, inhibitors, activators


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