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Pyronin Y

Cat. No. M13798
Pyronin Y Structure

Pyronine G; C.I. 45005

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100mg USD 55  USD55 In stock
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Quality Control & Documentation
Biological Activity

Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells.

Chemical Information
Molecular Weight 302.8
CAS Number 92-32-0
Solubility (25°C) DMSO 25 mg/mL
Water 4 mg/mL
Storage -20°C, protect from light, dry, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Burcu Meryem Beşer, et al. Luminescence. Photophysical and photodynamic properties of Pyronin Y in micellar media at different temperatures

[2] Burcu Meryem Beşer, et al. Luminescence. Photophysics and photodynamics of Pyronin Y in n-alcohols

[3] Ayad Eddaoudi, et al. Methods Mol Biol. Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining

[4] Kang Ho Kim, et al. Curr Protoc Mol Biol. Assaying Cell Cycle Status Using Flow Cytometry

[5] Allison Mayle, et al. Cytometry A. Flow cytometry analysis of murine hematopoietic stem cells

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Keywords: Pyronin Y, Pyronine G; C.I. 45005 supplier, Fluorescent Dye, inhibitors, activators

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