All AbMole products are for research use only, cannot be used for human consumption.
Phalloidin-Fluor 488 Conjugate selectively binds to F-actins with much higher photostability than the fluorescein-phalloidin conjugates.
Ex (nm) 491, Em (nm) 516.
Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution.
| Solubility (25°C) | DMSO |
| Storage | -20°C, protect from light, sealed |
| Related Fluorescent Dye Products |
|---|
| pH Fluorescent Probe Red 600, SE
pH Fluorescent Probe Red 600, SE is a pH-sensitive fluorescent dye, the fluorescence intensity changes significantly with changes in the pH of the environment. pH Fluorescent Probe Red 600, SE is weakly fluorescent outside the cells, but its fluorescence is significantly enhanced in acidic compartments (such as phagosomes, lysosomes and endosomes). It can be used for multiplexing cellular functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. Ex (nm) 576, Em (nm) 597 |
| DSPE-Rhodamine
DSPE-Rhodamine is formed by the conjugation of DSPE with the Rhodamine fluorescent dye. DSPE possesses a hydrophobic lipid tail and a hydrophilic head group, while Rhodamine is a red fluorescent dye. DSPE-Rhodamine retains the lipid properties of DSPE while also imparting fluorescent labelling capabilities. As a key component of delivery systems, DSPE-Rhodamine enhances targeting and bioavailability. Its lipid properties facilitate the penetration of molecules through cell membranes, while its fluorescent properties enable tracking of distribution and dynamic changes within the body. |
| LD540
LD540 is a novel high-sensitivity lipophilic dye modified with BODIPY fluorescent groups, designed for precise labelling and imaging of lipid droplets. LD540 exhibits excellent photostability and an optimal fluorescence spectrum, making it compatible with various commonly used fluorescent dyes (such as DAPI and Alexa Fluor 647), thereby supporting multi-colour imaging requirements. Additionally, LD540 is suitable for both fixed and live cells and can label ultra-small lipid droplets. |
| PDMPO
PDMPO (Yellow/Blue DND-160) is a ratiometric probe for the determination of lysosomal pH for fluorescence imaging. PDMPO exhibits pH-dependent dual excitation and dual emission peaks. PDMPO produces blue fluorescence (Ex/Em=329 nm/440 nm) in weakly acidic organelles, and in more acidic lysosomes it becomes yellow fluorescence (Ex/Em=384 nm/540 nm). |
| Copper probe CF4
Copper probe CF4 (Copper fluor CF4) is a Cu+-specific fluorescent probe based on a rhodol dye scaffold. Copper probe CF4 (Copper fluor CF4) has high copper selectivity with a Kd value of 2.9×10-13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper probe CF4 (Copper fluor CF4) is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission). Copper probe CF4 (Copper fluor CF4) can be used to study colon cancer. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
