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Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins. Most amino acids can be hydrolyzed and react with ninhydrin. The amino acids are then quantified colorimetrically after separation by chromatography. Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product: diketohydrindylidene-diketohydrindamine.
| Molecular Weight | 178.14 |
| Formula | C9H6O4 |
| CAS Number | 485-47-2 |
| Solubility (25°C) | DMSO ≥ 30 mg/mL Water 20 mg/mL (ultrasonic and warming) |
| Storage | 2-8°C, protect from light, sealed |
[5] Darren B Hansen, et al. Chem Soc Rev. The development of novel ninhydrin analogues
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