Mitotracker red cmxros is a red fluorescent dye used for staining mitochondria of living cells. Mitotracker red cmxros is a cell permeable x-rosamine derivative containing a weakly thiol reactive chloromethyl functional group that marks mitochondria. The maximum excitation / emission wavelength is 579 / 599nm- At 20 ℃, it shall be sealed and dried in a dark and moisture-proof manner. This product is an oxidized red fluorescent dye. It can be passively transported through the cell membrane and directly accumulated on the active mitochondria by simply incubating the cells. The accumulation of this dye depends on the membrane potential. Once the mitochondria are stained, they can also be fixed (aldehyde fixative such as formaldehyde) and permeabilized (aldehyde detergent such as Triton X-100) according to the needs of subsequent experiments, and the probe is still maintained in the cell. Because the red fluorescence of mitotracker red cmxros has good resolution with other green fluorescence probes, this product is suitable for double standard experiments.
| Molecular Weight | 531.5236 |
| Formula | C32H32Cl2N2O |
| CAS Number | 167095-09-2 |
| Solubility (25°C) | Soluble in DMSO |
| Storage | -20°C, protect from light, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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