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Mito Red

Cat. No. M22484
Mito Red Structure
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Biological Activity

Mito Red is a cell membrane permeable rhodamine based dye. It localizes mitochondria, and emits red fluorescence. The interaction of Mito Red with mitochondria depends of the membrane potential of the mitochondria. Mitochondria can be stained with 20-200 nM of Mito Red. MitoRed (Mito Red), a vital dye and mitochondrial stain, is used to indicate the status of mitochondria under various physiological and physical conditions.

Chemical Information
Molecular Weight 637.16
Formula C38H37ClN2O5
CAS Number 1021902-10-2
Solubility (25°C) DMSO
Storage 2-8°C, protect from light, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Simone Caielli, et al. Cell. Erythroid mitochondrial retention triggers myeloid-dependent type I interferon in human SLE

[2] Meysam Yazdankhah, et al. Autophagy. BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes

[3] Toshihiko Mayumi, et al. J Hepatobiliary Pancreat Sci. Tokyo Guidelines 2018: management bundles for acute cholangitis and cholecystitis

[4] Harumi Gomi, et al. J Hepatobiliary Pancreat Sci. Tokyo Guidelines 2018: antimicrobial therapy for acute cholangitis and cholecystitis

[5] Masamichi Yokoe, et al. J Hepatobiliary Pancreat Sci. Tokyo Guidelines 2018: diagnostic criteria and severity grading of acute cholecystitis (with videos)

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