L-NAME hydrochloride

Cat. No. M2818

Synonym:

NG-Nitroarginine methyl ester hydrochloride

Size	Price	Availability
Free Sample (0.5-1 mg)	USD 0	In stock
100mg	USD 40 USD40	In stock
500mg	USD 85 USD85	In stock

Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control

Current batch:
Purity 99.43%
COA
MSDS
H-NMR
HPLC

Product Information

Biological Activity

L-NAME hydrochloride is a nonselective inhibitor of nitric oxide synthetases (NOS) for nNOS (bovine), eNOS (human), and iNOS (murine), with K_i of 15 nM, 39 nM and 4.4 μM, respectively.

Product Citations

Oxid Med Cell Longev. 2021 Jun 14;2021:6662225.

Impaired Autophagy Induced by oxLDL/ β 2GPI/anti- β 2GPI Complex through PI3K/AKT/mTOR and eNOS Signaling Pathways Contributes to Endothelial Cell Dysfunction
L-NAME hydrochloride purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Oxid Med Cell Longev (2021). Figure 5. (Abmole Bioscience, USA)
	Method	Cell Culture and Treatment
	Cell Lines	HUVECs and bEnd.3 cells
	Concentrations	100 μM
	Incubation Time	4 h
	Results	We found that LY294002, AZD5363, rapamycin, and L-NAME pretreatment could significantly upregulate the expression of LC3-II, while downregulating the expression of p62 compared with the oxLDL/β2GPI/anti-β2GPI complex alone group

Protocol

Cell Experiment
Cell lines	H9C2 cells
Preparation method	H9C2 cells were exposed to hypoxia (2%O2; 4 h) or normoxic medium (21%O2; 4 h). After 4 h, culture medium was replaced with reoxygenated medium (21%O2 2 h) for H/R and normoxia controls, or hypoxic medium (2%O2; 2 h) in the presence and absence of 50 µM MnTBap or 100 µM L-NAME and then further incubated for 2 h in the presence of MTT reagent.
Concentrations	100 µM
Incubation time	2 h

Animal Experiment
Animal models	Male Sprague-Dawley rats
Formulation	distilled water
Dosages	40 mg/kg/day
Administration	i.v.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m²)	0.007	0.025	0.15	0.05	0.02	0.5
K_m factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B K_m
	Animal A K_m

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information

Molecular Weight	269.69
Formula	C₇H₁₅N₅O₄.HCl
CAS Number	51298-62-5
Purity	99.43%

Solubility	Water: ≥ 30 mg/mL
Storage	at -20°C

References

[1] Berkban T, et al. Nutrients. Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47phox Expression in Rats.

[2] Kolamunne RT, et al. Redox Biol. Nrf2 activation supports cell survival during hypoxia and hypoxia/reoxygenation in cardiomyoblasts; the roles of reactive oxygen and nitrogen species.

Related NOS Products
Amcinonide Amcinonide (Cyclocort, CL-34699) is an inhibitor of nitric oxide (NO) release from activated microglia with IC50 of 3.38 nM.
Bendazol Bendazol (2-Benzylbenzimidazole, Dibazol, Dibazole, Bendazole, Tromasedan) is a hypotensive drug that enhances NO synthase activity in renal glomeruli and collecting tubules. Bendazol inhibits the progression of form-deprivation myopia (FDM) and suppresses the upregulation of HIF-1α.
Syzalterin Syzalterin is an inhibitor of NO production with an IC50 of 1.87 μg/mL.
L-NIO dihydrochloride L-NIO dihydrochloride is a potent, non-selective and NADPH-dependent nitric oxide synthase (NOS) inhibitor, with K_is of 1.7, 3.9, 3.9 μM for neuronal (nNOS), endothelial (eNOS), and inducible (iNOS), respectively.
L-NIL L-NIL is an inducible NO synthase inhibitor, with an IC50 of 3.3 μM for miNOS.

Catalog

Signaling Pathways

Product Type