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L-NAME hydrochloride

Cat. No. M2818
L-NAME hydrochloride Structure
Synonym:

NG-Nitroarginine methyl ester hydrochloride

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
100mg USD 40  USD40 In stock
500mg USD 85  USD85 In stock
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Quality Control
Biological Activity

L-NAME hydrochloride is a nonselective inhibitor of nitric oxide synthetases (NOS) for nNOS (bovine), eNOS (human), and iNOS (murine), with Ki of 15 nM, 39 nM and 4.4 μM, respectively.

Product Citations
Customer Product Validations & Biological Datas
Source Oxid Med Cell Longev (2021). Figure 5. (Abmole Bioscience, USA)
Method Cell Culture and Treatment
Cell Lines HUVECs and bEnd.3 cells
Concentrations 100 μM
Incubation Time 4 h
Results We found that LY294002, AZD5363, rapamycin, and L-NAME pretreatment could significantly upregulate the expression of LC3-II, while downregulating the expression of p62 compared with the oxLDL/β2GPI/anti-β2GPI complex alone group
Protocol
Cell Experiment
Cell lines H9C2 cells
Preparation method H9C2 cells were exposed to hypoxia (2%O2; 4 h) or normoxic medium (21%O2; 4 h). After 4 h, culture medium was replaced with reoxygenated medium (21%O2 2 h) for H/R and normoxia controls, or hypoxic medium (2%O2; 2 h) in the presence and absence of 50 µM MnTBap or 100 µM L-NAME and then further incubated for 2 h in the presence of MTT reagent.
Concentrations 100 µM
Incubation time 2 h
Animal Experiment
Animal models Male Sprague-Dawley rats
Formulation distilled water
Dosages 40 mg/kg/day
Administration i.v.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 269.69
Formula C7H15N5O4.HCl
CAS Number 51298-62-5
Purity 99.43%
Solubility Water: ≥ 30 mg/mL
Storage at -20°C
References

[1] Berkban T, et al. Nutrients. Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47phox Expression in Rats.

[2] Kolamunne RT, et al. Redox Biol. Nrf2 activation supports cell survival during hypoxia and hypoxia/reoxygenation in cardiomyoblasts; the roles of reactive oxygen and nitrogen species.

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  Catalog
Abmole Inhibitor Catalog




Keywords: L-NAME hydrochloride, NG-Nitroarginine methyl ester hydrochloride supplier, NOS, inhibitors

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