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Kyoto probe 1 is a hiPSCs fluorescent probe. Kyoto probe 1 selectively labels human pluripotent stem cells. Kyoto probe 1 is primarily explained by the distinct expression patterns of ATP-binding cassette (ABC) transporters by hiPS cells and differentiated cells. Kyoto probe 1 (KP-1) rapidly distinguishes between human ES/iPS (hES/iPS) cells and their differentiated cells.
KP-1 is a fluorescent imaging probe that detects live human pluripotent stem cells in distinction from for detection of differentiated cells. KP-1 is permeable through the cell membrane and localize on the mitochondrial membrane by adding the probe to the culture medium.KP-1 is considered to be eliminated from the inside of the differentiated cells by the ABCtransporters which do not work in the pluripotent stem cells.
515 nm is suited for excitation wavelength. The wavelength of maximum emission is around 529 nm.
Procedure (for reference only)
1) Plate human iPS cells on the layer of the SNL feeder cells. Colonies of iPS cell are formed about 5 days after the plating.
Caution : Glass bottom dish etc. are recommended as cell culture dish, because it shows a low autofluorescence.
2) Dissolve 10 μg of KP-1 (1 vial) in 4.8 μL of DMSO to prepare 5 mM stock solution.
Caution : You are able to solve KP-1 in PBS (pH7.4) at the concentration of 0.25 mg/mL or below, if you do not want to use DMSO.
3) Dilute the DMSO stock solution with iPS culture medium to 2 µM cell stain solution.
4) Add stain solution to the dish and incubate for 3 hours.
5) Replacing the buffer to the iPS culture medium or HBSS and observe the cells using a fluorescence microscopy.
| Molecular Weight | 418.34 |
| Formula | C21H14F4N2O3 |
| CAS Number | 2088021-81-0 |
| Solubility (25°C) | DMSO 5 mM |
| Storage | -20°C, protect from light, dry, sealed |
[2] Nao Hirata, et al. Cell Rep. A chemical probe that labels human pluripotent stem cells
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