Hoechst 34580 (HOE 34580) is related to important small furrow binding dsDNA selective dyes Hoechst 33258 and Hoechst 33342, with a maximum excitation/emission wavelength of 392nm/440 nm (after binding to DNA), emitting blue fluorescence. For observation of nuclei, nucleic acids, stored at -25°C, away from light.
Molecular Weight | 451.57 |
Formula | C27H29N7 |
CAS Number | 23555-00-2 |
Solubility (25°C) | Water ≥ 40 mg/mL |
Storage | -25°C, protect from light, dry, sealed |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] Eugnia Nogueira, et al. Biomacromolecules. Peptide Anchor for Folate-Targeted Liposomal Delivery
[5] Wanhua Lei, et al. Chemphyschem. Host-guest interaction of Hoechst 34580 and Cucurbit[7]uril
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