Hederagenin is a triterpenoid saponin. It can inhibit LPS-stimulated expression of iNOS, COX-2, and NF-κB. Hederagenin may be a promising candidate for human colon cancer studies. Hederagenin could significantly inhibit the viability of Lovos cells in a concentration and time-dependent manner with IC50 of 1.39μM at 24 h and 1.17μM at 48 H. Hederagenin could also induce apoptosis by nuclear changes characterized by Hirst 33342 nuclear stainining fluorescence microscopy. Dcfh-da fluorescence staining and flow cytometry showed that Hederagenin could significantly increase the production of ROS in LoVo cells. Real-time PCR results showed that Hederagenin induced up-regulation of Bax expression and down-regulation of Bcl-2, Bcl-XL and Survivin expression. Western blotting showed that Hederagenin reduced the expression of apoptosis-related proteins Bcl-2, Procaspase-9, procaspase-3 and Polyadp-Ribosepolymerase (PARP). Increased the expression of Bax, caspase-3 and caspase-9. However, there was no significant change in caspase-8.
| Molecular Weight | 472.70 |
| Formula | C30H48O4 |
| CAS Number | 465-99-6 |
| Solubility (25°C) | DMSO 45 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related COX Products |
|---|
| Oxyphenbutazone
Oxyphenbutazone is an orally active non-selective COX inhibitor. Oxyphenbutazone selectively kills non-replicating Mycobaterium tuberculosis. |
| Birch triterpenes
Birch triterpenes is a cyclooxygenase-2 (COX-2) inhibitor that can be used in studies related to epidermolysis bullosa. |
| Naproxcinod
Naproxcinod is a first-of-its-kind (first-in-class) cyclooxygenase-inhibiting nitric oxide donors (CINODs) with analgesic and anti-inflammatory activity for studies related to osteoarthritis and inflammation. |
| Hamaudol
Hamaudol is a chromone isolated from Saposhnikovia divaricata. |
| (±)-Catechin
(±)-Catechin (rel-Cianidanol) is the racemate of Catechin. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.
