AbMole Freund′s Adjuvant, Complete is formulated with low endotoxin, high-purity paraffin oil and mannide monooleate and Mycobacterium tuberculosis (heat killed and dried), without preservatives. It is one of the most commonly used immune adjuvants in the preparation of antibodies for animal immunization.
The mycobacteria in Complete Freund′s adjuvant attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, Complete Freund′s Adjuvant is used for initial injections and Incomplete Freund′s Adjuvant for subsequent boosts.
| Solubility (25°C) | Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion. |
| Storage | 2-8°C, protect from light, sealed. Do not freeze! |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] David D Brand, et al. Nat Protoc. Collagen-induced arthritis
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| Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat
Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) is a minor component of CNS myelin. |
| PLP (139-151)
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| G6PI 325-339 (human)
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| Heparin sodium salt (MW 15kDa)
Heparin sodium salt (MW 15kDa) is a heparin polymer with a molecular weight of 15kDa.Heparin sodium salt is an anticoagulant that reversibly binds to antithrombin III (ATIII), thereby accelerating the rate of inactivation of Thrombin factor IIa and factor Xa by ATIII. Heparin sodium salt is used for blood collection and plasma preparation in experimental animals and for the construction of parenteral nutrition models in mice. |
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