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FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site, which is used for antibody-mediated identification and purification of recombinant proteins. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.
| Molecular Weight | 1012.97 |
| Formula | C41H60N10O20 |
| CAS Number | 98849-88-8 |
| Solubility (25°C) | Water ≥ 90 mg/mL |
| Storage | -20°C, dry, protect from light, sealed |
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