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FITC

Cat. No. M9631

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FITC Structure
Synonym:

5-FITC; 5-Isothiocyanato fluorescein; FITC isomer I

Size Price Availability Quantity
100mg USD 45  USD45 In stock
500mg USD 90  USD90 In stock
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Quality Control & Documentation
Biological Activity

FITC (Fluorescein Isothiocyanate, 5-FITC) is an amine-reactive reagent for the FITC labeling of proteins, microsequencing of proteins, and peptides. The isothiocyanate group of FITC can be combined with amino, sulfhydryl, imidazole, tyrosyl, carbonyl and other groups on the protein, so as to achieve protein labeling including antibodies and lectins. In addition to its use as a protein marker, FITC can also be used as a fluorescent protein tracer to rapidly identify pathogens by labeling antibodies, or for microsequencing of proteins and peptides (HPLC). The maximum excitation wavelength of FITC is 494 nm. Once excited, it fluoresces yellow-green at a maximum emission wavelength of 520 nm.

Chemical Information
Molecular Weight 389.38
Formula C21H11NO5S
CAS Number 3326-32-7
Solubility (25°C) DMSO 40 mg/mL
Storage 2-8°C, protect from light, dry, sealed
References

[1] Xiaojun Hu, et al. Cell membrane-coated gold nanoparticles for apoptosis imaging in living cells based on fluorescent determination

[2] Lijuan Sun, et al. Preparation and evaluation of fluorescent poly(p-phenyleneethylene) covalently coated microspheres with reactive sites for bioconjugation

[3] Przemyslaw Zarski, et al. Super Stable Fluorescein Isothiocyanate Isomer I Monolayer for Total Internal Reflection Fluorescence Microscopy

[4] E Zhang, et al. Use of fluorescein isothiocyanate isomer I to study the mechanism of intestinal absorption of fucoidan sulfate in vivo and in vitro

[5] Arvind Chopra. Biotinylated bovine serum albumin linked to gadolinium diethylenetriaminepentaacetic acid

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Keywords: FITC, 5-FITC; 5-Isothiocyanato fluorescein; FITC isomer I supplier, Fluorescent Dye, inhibitors, activators

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