All AbMole products are for research use only, cannot be used for human consumption.
FeRhoNox-1, also known as RhoNox-1, is an active fluorescent probe that specifically detects unstable iron(II) ions (Fe2+). Once reacted with Fe2+, an orange (red) fluorescent product is irreversibly generated. (Absmax= 540nm, FLmax= 575nm). FeRhoNox-1 is permeable to cell membranes and highly selective, which is suitable for the detection of Fe2+ in living cells and tends to be localized in the Golgi apparatus.
Protocol (for reference only)
1. Preparation of RhoNox-1 working solution
1.1 Preparation of the stock solution
Dissolve 50 μg RhoNox-1 in 110 μL DMSO to obtain 1 mM of stock solution.
1.2 Preparation of RhoNox-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of RhoNox-1 working solution according to the actual situation.
2. Cell staining (6-well plate)
2.1 Suspension cells
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.
| Solubility (25°C) | DMSO |
| Storage | -20°C, protect from light, dry, sealed |
| Related Fluorescent Dye Products |
|---|
| ER-Tracker Green
ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide, highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation. |
| DAR-2
DAR-2 is a sensitive fluorescent NO probe. |
| ZnAF-2 DA
ZnAF-2 DA is a fluorescent Zn2+ probe suitable for detection of Zn2+ (after esterase hydrolysis). |
| ZnAF-1
ZnAF-1, a fluorescein-based zinc sensor containing the N,N-bis(2-pyridylmethyl)ethylenediamine chelating unit, can be used for Zn2+ detection. |
| Fluorescein octadecyl ester
Fluorescein octadecyl ester is a Lipophilic fluorescent pH indicator. Fluorescein octadecyl ester is a lipophilic fluorescent reagent is immobilized in a plasticized PVC membrane. Fluorescein octadecyl ester can reversibly recognize alcohol molecules and can be used to determine the concentration of ethanol in alcoholic drinks. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
