All AbMole products are for research use only, cannot be used for human consumption.
FeRhoNox-1, also known as RhoNox-1, is an active fluorescent probe that specifically detects unstable iron(II) ions (Fe2+). Once reacted with Fe2+, an orange (red) fluorescent product is irreversibly generated. (Absmax= 540nm, FLmax= 575nm). FeRhoNox-1 is permeable to cell membranes and highly selective, which is suitable for the detection of Fe2+ in living cells and tends to be localized in the Golgi apparatus.
Protocol (for reference only)
1. Preparation of RhoNox-1 working solution
1.1 Preparation of the stock solution
Dissolve 50 μg RhoNox-1 in 110 μL DMSO to obtain 1 mM of stock solution.
1.2 Preparation of RhoNox-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of RhoNox-1 working solution according to the actual situation.
2. Cell staining (6-well plate)
2.1 Suspension cells
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.
| Molecular Weight | 458.55 |
| Formula | C28H30N2O4 |
| Solubility (25°C) | DMSO |
| Storage | -20°C, protect from light, dry, sealed |
| Related Fluorescent Dye Products |
|---|
| DASPEI
DASPEI is a cationic styrenyl mitochondrial dye. DASPEI has excitation and emission wavelength at 550/573 nm, which has good light chromogenic property. DASPEI can stain mitochondria in living cells with good labeling property. DASPEI can also be used to stain presynaptic nerve endings independently of neuronal activity. |
| Zinquin ethyl ester
Zinquin ethyl ester is a fluorescent probe of cytosolic zinc ion. Zinquin ethyl ester is able to penetrate cell membranes and is lipophilic and zinc-sensitive. Zinquin ethyl ester can combine with Zn2+ in the cell to produce blue fluorescence. |
| HKOH-1r
HKOH-1r is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively. |
| ATP-Red 1
ATP-Red 1 is a multisite-binding switchable fluorescent probe, and it can selectively and rapidly responds to intracellular concentrations of ATP in living cells. Live-cell imaging indicated that ATP-Red 1 mainly localized to mitochondria with good biocompatibility and membrane penetration. ATP-Red 1 is a useful tool for investigating ATP-relevant biological processes. |
| (±)-ANAP hydrochloride
(±)-ANAP hydrochloride is the amino acid analog of prodan, acts as a fluorescent probes, and enhances environmental sensitivity with comparable or increased brightness. (±)-ANAP hydrochloride is sensitive to polarity with changes in intensity and emission wavelength. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
