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Ethidium Homodimer 1 (EthD-1) is a high-affinity fluorescent nucleic acid dye that binds ssDNA, dsDNA, RNA, oligonucleotides and triple helix DNA. Once it is combined with DNA, the fluorescence is significantly enhanced (>30 times), and bright red fluorescence is invented (the maximum excitation/emission wavelength is about 528/617nm). Since EthD-1 carries a strong positive charge and cannot penetrate intact/living cell membranes, it can be used to determine cell viability. Based on the above characteristics, EthD-1 can be used in combination with Calcein AM to stain dead and live cells respectively to determine cell viability and toxicity. EthD-1 is a dead cell specific dye in all cell types (including mammals, bacteria and yeast).
| Molecular Weight | 856.75 |
| Formula | C46H50Cl4N8 |
| CAS Number | 61926-22-5 |
| Solubility (25°C) | DMSO 2 mM DMF |
| Storage | 2-8°C, protect from light, dry, sealed |
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