Free shipping on all orders over $ 500

Dispase II

Cat. No. M21672
Dispase II Structure
Size Price Availability Quantity
50mg USD 60  USD60 In stock
100mg USD 90  USD90 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures.

Enzyme activity: DispaseII ≥0.8 U/mg (37°C, using casein as substrate, pH 7.5)


Preparation Note

Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.

Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.

Working concentration: 0.6 to 2.4 U/ml

Preparation of stock and working solutions:

To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.


Storage conditions (working solution): -20 °C

The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.

Chemical Information
CAS Number 42613-33-2
Solubility (25°C) 50 mM HEPES/KOH pH 7.4 150 mM NaCl
Storage Stable for 6-12 months after receipt, store at 2-8°C, dry, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Beln Calvo, et al. IBRO Rep. Dissociation of neonatal and adult mice brain for simultaneous analysis of microglia, astrocytes and infiltrating lymphocytes by flow cytometry

[2] Jae-Kyung Lee, et al. Methods Mol Biol. Microglia isolation from adult mouse brain

[3] Mark Tomishima. Splitting hPSCs with Dispase

[4] Laurence S Lim, et al. Mol Vis. Effect of dispase denudation on amniotic membrane

[5] S J Spurr, et al. Invest Ophthalmol Vis Sci. Isolation of corneal epithelium with Dispase II or EDTA. Effects on the basement membrane zone

Related Cell Culture Products
Dipotassium hydrogen phosphate

Dipotassium hydrogen phosphate is a highly water-soluble salt which is often used as a fertilizer, food additive and buffering agent.

L-Arabinose

L-Arabinose is the naturally occurring isomer and is a constituent of plant polysaccharides. Most bacteria contain an inducible arabinose operon that codes for a series of enzymes and transporters that allows L-arabinose to be used as the sole carbon source in microbial culture.

PCL-PVAc-PEG

PCL-PVAc-PEG is a polymer that can be used in studies related to hematopoietic stem cell expansion culture.

Butyzamide

Butyzamide is a novel, potent, orally active activator of the non-peptidic thrombopoietin receptor (Mpl) that specifically reacts with human Mpl and activates the same signaling pathways as thrombopoietin. In addition, Butyzamide can be used for the expansion of human hematopoietic stem cells.

D-Glucosemonohydrate

D-(+)-glucose monohydrate is a common natural sugar involved in processes such as glycosylation, energy production, and the formation of glycans, which are structural components of cells. d-(+)-glucose monohydrate can also cause glycation, which is a harmful process in cells. It is used as a supplement for many cellular processes and cell culture.

  Catalog
Abmole Inhibitor Catalog




Keywords: Dispase II supplier, Cell Culture, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.