All AbMole products are for research use only, cannot be used for human consumption.
Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures.
Enzyme activity: DispaseII ≥0.8 U/mg (37°C, using casein as substrate, pH 7.5)
Preparation Note
Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Working concentration: 0.6 to 2.4 U/ml
Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.
Storage conditions (working solution): -20 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
CAS Number | 42613-33-2 |
Solubility (25°C) | 50 mM HEPES/KOH pH 7.4 150 mM NaCl |
Storage | Stable for 6-12 months after receipt, store at 2-8°C, dry, sealed |
[2] Jae-Kyung Lee, et al. Methods Mol Biol. Microglia isolation from adult mouse brain
[3] Mark Tomishima. Splitting hPSCs with Dispase
[4] Laurence S Lim, et al. Mol Vis. Effect of dispase denudation on amniotic membrane
Related Cell Culture Products |
---|
Collagen Type I, Bovine (300 kDa)
Bovine Collagen I is a purified stromal extracellular matrix protein that can promtes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture. |
Collagen Type I, Bovine
Bovine Collagen I is a purified stromal extracellular matrix protein that can promtes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture. |
O4I3
O4I3 is a potent and selective inhibitor of the KDM5A (JARID1A) isoform of H3K4 demethylase, with an IC50 value of 0.15 nM.O4I3 effectively induces OCT4 expression and enhances OSKM-induced pluripotent stem cell reprogramming efficiency (50 nM) of human primary fibroblasts by preventing KDM5A from interfering with the enrichment of H3K4Me3 on the OCT4 promoter. iPSC) reprogramming efficiency (50 nM). |
Glycogen (from oyster)
Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. Oyster glycogen has been used to induce inflammation and to promote extravasation of granulocytes and neutrophils in animal models after intraperitoneal injection. Glycogen also acts as a vascular permeability probe. |
L-Alanyl-L-glutamine, for cell culture
L-Alanyl-L-glutamine, for cell culture is cell culture grade proglutide, a stabilized form of L-glutamine. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.