Diphenylterazine is a bioluminescence agent. Diphenylterazine alone yields very little background, leading to excellent signal-to-background ratios.
To make a stock solution for Diphenylterazine, a premixture is prepared by dissolving 17.6 mg of L-ascorbic acid in 10 mL ethanol and 10 mL 1,2-propanediol firstly; next, 1 mg of Diphenylterazine is dissolved in 88 µL of the premix, resulting in a 30 mM DTZ stock solution containing 5 mM L-ascorbic acid.
Diphenylterazine (0.3 μmol/mouse [1.13 mg/ml; 100 ul/mouse]; i.v.) treatment can track tumor growth in a xenograft NU/J mouse model.
DMSO can inactivate Diphenylterazine's activity
| Molecular Weight | 377.44 |
| Formula | C25H19N3O |
| CAS Number | 344940-63-2 |
| Solubility (25°C) | DMF 10 mg/mL |
| Storage | -20°C, protect from light, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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