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Chlorazol Black E

Cat. No. M25563
Chlorazol Black E Structure

Direct Black 38; Ferristatin II disodium

Size Price Availability Quantity
100mg USD 30  USD30 In stock
500mg USD 50  USD50 In stock
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Quality Control & Documentation
  • Purity: Biological Stain
  • COA
  • MSDS
Biological Activity

Chlorazol Black E is an azo dye. Chlorozol Black E is a chitin-specific stain and used to detect fungal filaments. Chlorazol Black E (Direct Black 38) can induce unscheduled DNA synthesis in liver and micronucleus in bone marrow of rats in vivo.

Chlorazol Black E (Direct Black 38) is slightly soluble in water for green light black, and adequate soluble in alcohol for green light blue-black. The strong sulfuric acid is a deep red in blue, diluted Purple dark reddish brown for to red light black precipitation; In nitric acid solution for yellow brown; In strong hydrochloric acid for dark red light black solution. For cellulose fiber dyeing, the dye absorbency is very good.

Chemical Information
Molecular Weight 781.73
Formula C34H25N9Na2O7S2
CAS Number 1937-37-7
Form Solid
Solubility (25°C) Water 5 mg/mL
DMSO 10 mg/mL
Storage 4°C, protect from light, dry, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Yoshie Nishida, et al. Jpn J Infect Dis. Genotyping Giardia intestinalis by Using DNA Extracted from Long-Term Preserved Human Specimens Stained with Chlorazol Black E

[2] Philip A Thomas, et al. Can J Ophthalmol. Rapid detection of Acanthamoeba cysts in corneal scrapings by chlorazol black E staining

[3] Philip Aloysius Thomas, et al. Am J Ophthalmol. Use of chlorazol black E mounts of corneal scrapes for diagnosis of filamentous fungal keratitis


[5] L Kass. Am J Clin Pathol. Staining of granulocytic cells by Chlorazol black E

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Keywords: Chlorazol Black E, Direct Black 38; Ferristatin II disodium supplier, Fluorescent Dye, inhibitors, activators

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