Bafilomycin A1

Biological Activity

Bafilomycin A1 (BafA1) is a toxic macrolide antibiotic derived from Streptomyces griseus. Bafilomycin A1 inhibits the short circuit current induced by the outer mantle epithelium (OME). The IC50 and maximum inhibition dose of Bafilomycin A1 are 0.17 μM and 0.5 μM, respectively. Bafilomycins are specific inhibitors of vacuolar-type H+-ATPase. (V-ATPase). The most used bafilomycin is bafilomycin A1. This is a useful tool as it can prevent the re-acidification of synaptic vesicles once they have undergone exocytosis.

Product Citations

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  Oxid Med Cell Longev. 2022 Jan 25;2022:8096009.

  Interaction between TRPML1 and p62 in Regulating Autophagosome-Lysosome Fusion and Impeding Neuroaxonal Dystrophy in Alzheimer's Disease
  Bafilomycin A1 purchased from AbMole

• 

  Transl Psychiatry. 2021 Aug 5;11(1):421.

  BRUCE silencing leads to axonal dystrophy by repressing autophagosome-lysosome fusion in Alzheimer's disease
  Bafilomycin A1 purchased from AbMole

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  Mol Immunol. 2020 Jan;117:12-19.

  Toll-like receptor 4 knockout protects against diabetic-induced imbalance of bone metabolism via autophagic suppression.
  Bafilomycin A1 purchased from AbMole

Chemical Information

Molecular Weight	622.83
Formula	C35H58O9
CAS Number	88899-55-2
Solubility (25°C)	DMSO 10 mM
Storage	-20°C, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Oliveira PF, et al. Comp Biochem Physiol A Mol Integr Physiol. H+-ATPase of crude homogenate of the outer mantle epithelium of Anodonta cygnea.

[2] al-Fifi ZI, et al. Insect Biochem Mol Biol. Characterization of ATPases of apical membrane fractions from Locusta migratoria Malpighian tubules.