Asaraldehyde (Asaronaldehyde) is a selective COX-2 inhibitor. Asaraldehyde (Asaronaldehyde) was found to be the most abundant constituent, but was totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, asaraldehyde (Asaronaldehyde) was produced in both cultured broths. Asaraldehyde (Asaronaldehyde) showed 3.32% of prostaglandin H endoperoxide synthase-1 (COX-1) inhibitory activity and 52.69% prostaglandin H endoperoxide synthase-2 (COX-2) inhibitory activity, respectively at 100 mg/ml. 2,4,5-trimethoxybenzaldehyde showed selectivity towards COX-2 enzyme inhibition at 100 ug/ml. The COX-2/COX-1 ratio for compound 1 was 17.68 at 100 ug/ml compared to solvent control.
Cell Experiment | |
---|---|
Cell lines | 3T3-L1 cells |
Preparation method | Seeding 3T3-L1 cells in 96-well plates at a concentration of 104 /well. Twenty-four hours later, treating the cells with 100 μg/mL of Asaraldehyde for 24 hours or for the whole 8-day differentiation period. Also, testing fully differentiated adipocytes with 100 μg/mL of Asaraldehyde for 24 hours-72 hours to test the cytotoxicity. Culturing cells with MTT at a final concentration of 0.5 mg/mL for another 4 hours at the end of treatment. The purple MTT formazan is dissolved by DMSO and the absorbance at 570 nm is taken with a spectrophotometer. The absorbance is proportional to the viability of adipocytes |
Concentrations | 100 μg/mL |
Incubation time | 24 hours |
Animal Experiment | |
---|---|
Animal models | |
Formulation | |
Dosages | |
Administration |
Molecular Weight | 196.2 |
Formula | C10H12O4 |
CAS Number | 4460-86-0 |
Solubility (25°C) | DMSO 30 mg/mL |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Jian Zou, et al. Biotransformation of α-asarone by Alternaria longipes CGMCC 3.2875
[3] Cheol Hyeong Park, et al. Phenolic constituents of Acorus gramineus
Related COX Products |
---|
Birch triterpenes
Birch triterpenes is a cyclooxygenase-2 (COX-2) inhibitor that can be used in studies related to epidermolysis bullosa. |
Naproxcinod
Naproxcinod is a first-of-its-kind (first-in-class) cyclooxygenase-inhibiting nitric oxide donors (CINODs) with analgesic and anti-inflammatory activity for studies related to osteoarthritis and inflammation. |
Hamaudol
Hamaudol is a chromone isolated from Saposhnikovia divaricata. |
(±)-Catechin
(±)-Catechin (rel-Cianidanol) is the racemate of Catechin. |
PYZ18
PYZ18 is a selective COX-2 inhibitor with an IC50 of 7.07 μM. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.