Free shipping on all orders over $ 500

Anti-human CD3/CD28 beads for T cell

Cat. No. M11389
Anti-human CD3/CD28 beads for T cell Structure
Size Price Availability Quantity
1ml USD 710  USD710 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
  • Current batch:
  • Purity N/A
  • COA
  • MSDS
Biological Activity

Reactive species: Human
Endotoxin: <2EU/ml
Concentration: 2 x 108Beads/mL
Application: T lymphocyte

Bioactivity:  CD3/CD28 mab conjugated magnetic beads are mainly used for isolation, activation and in vitro amplification of human T cells. The use of 4.5-um superparamagnetic beads, matched to cell size, coupled with anti-CD3 and CD28 antibodies, provides the primary and co-stimulatory signals required for T cell activation and amplification. First, CD3/CD28 immunomagnetic beads were used for magnetic cell isolation, and CD3+ T cells were isolated and enriched from the obtained isolated products. After isolation, CD3+ T cells were cultured in the presence of magnetic beads. By binding anti-CD3 and anti-CD28 antibodies on immunomagnetic beads, the beads provide primary and costimulatory signals required for T cell activation and amplification. Activated T cells produce IL-2 (interleukin 2), GM-CSF (granulocyte macrophage stimulating factor), IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α), which play the role and function of T cells.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight
Formula
CAS Number
Purity N/A
Solubility N/A
Storage 2~8℃
Related Cytokines Products
DKK-1 (Mammalian,N-8His)

Recombinant Human Dickkopf-related protein 1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a 8His tag at the N-terminus.

FGF-basic, Rat(E. coli)

FGF-basic, Rat(E. coli) Expressed with an n-terminal Gly. FGF-basic (Pro10-Ser154) Accession # P13109. Bioactivity: ED50 < 0.25 ng/ mL, measured by 3T3 cell proliferation method, corresponding to > 4.0 × 106 units /mg.

TNF-α, Human (E. coli)

TNF-α, Human (E. coli) Protein structure: TNF-α (VAL77-LEU233) Accession # P01375. Bioactivity: ED50 < 30.0 pg/ mL, measured in cytotoxicity assay of L-929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D, corresponding specific activity > 3.3 × 107 units /mg.

BMP-2, Human(E. coli)

BMP-2, Human(E. coli) Protein structure: BMP-2 (GLN283-ARG396) Accession # P12643. Biological activity: Experiment 1: To determine its ability to induce alkaline phosphatase production in ATDC-5 cells. The ED50 for this effect is generally 0.07-0.2µg/ mL. Experiment 2: The ability of inducing ALKALINE phosphatase production in C2C12 cells was determined. The ED50 for this effect is usually 0.2-1µg/ mL.

SCF, Human (P. pastoris)

SCF, Human (P. pastoris) The protein was Expressed with an n-terminal Met. SCF (Glu26-Ala189) Accession # P21583-1. Biological activity: ED50 was < 2.0 ng/ mL by dose-dependent tF-1 cell stimulation, corresponding specific activity was 5 × 105IU /mg.

  Catalog
Abmole Inhibitor Catalog




Keywords: Anti-human CD3/CD28 beads for T cell supplier, Cytokines, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.