Anti-human CD3/CD28 beads for T cell

Biological Activity

Reactive species: Human
Endotoxin: <2EU/ml
Concentration: 2 x 108Beads/mL
Application: T lymphocyte

Bioactivity:  CD3/CD28 mab conjugated magnetic beads are mainly used for isolation, activation and in vitro amplification of human T cells. The use of 4.5-um superparamagnetic beads, matched to cell size, coupled with anti-CD3 and CD28 antibodies, provides the primary and co-stimulatory signals required for T cell activation and amplification. First, CD3/CD28 immunomagnetic beads were used for magnetic cell isolation, and CD3+ T cells were isolated and enriched from the obtained isolated products. After isolation, CD3+ T cells were cultured in the presence of magnetic beads. By binding anti-CD3 and anti-CD28 antibodies on immunomagnetic beads, the beads provide primary and costimulatory signals required for T cell activation and amplification. Activated T cells produce IL-2 (interleukin 2), GM-CSF (granulocyte macrophage stimulating factor), IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α), which play the role and function of T cells.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information