Reactive species: Human
Concentration: 2 x 108Beads/mL
Application: T lymphocyte
Bioactivity: CD3/CD28 mab conjugated magnetic beads are mainly used for isolation, activation and in vitro amplification of human T cells. The use of 4.5-um superparamagnetic beads, matched to cell size, coupled with anti-CD3 and CD28 antibodies, provides the primary and co-stimulatory signals required for T cell activation and amplification. First, CD3/CD28 immunomagnetic beads were used for magnetic cell isolation, and CD3+ T cells were isolated and enriched from the obtained isolated products. After isolation, CD3+ T cells were cultured in the presence of magnetic beads. By binding anti-CD3 and anti-CD28 antibodies on immunomagnetic beads, the beads provide primary and costimulatory signals required for T cell activation and amplification. Activated T cells produce IL-2 (interleukin 2), GM-CSF (granulocyte macrophage stimulating factor), IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α), which play the role and function of T cells.
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
|Related Cytokines Products|
Recombinant Human Dickkopf-related protein 1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a 8His tag at the N-terminus.
|FGF-basic, Rat（E. coli）
FGF-basic, Rat（E. coli） Expressed with an n-terminal Gly. FGF-basic (Pro10-Ser154) Accession # P13109. Bioactivity: ED50 < 0.25 ng/ mL, measured by 3T3 cell proliferation method, corresponding to > 4.0 × 106 units /mg.
|TNF-α, Human (E. coli)
TNF-α, Human (E. coli) Protein structure: TNF-α (VAL77-LEU233) Accession # P01375. Bioactivity: ED50 < 30.0 pg/ mL, measured in cytotoxicity assay of L-929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D, corresponding specific activity > 3.3 × 107 units /mg.
|BMP-2, Human（E. coli）
BMP-2, Human（E. coli） Protein structure: BMP-2 (GLN283-ARG396) Accession # P12643. Biological activity: Experiment 1: To determine its ability to induce alkaline phosphatase production in ATDC-5 cells. The ED50 for this effect is generally 0.07-0.2µg/ mL. Experiment 2: The ability of inducing ALKALINE phosphatase production in C2C12 cells was determined. The ED50 for this effect is usually 0.2-1µg/ mL.
|SCF, Human (P. pastoris)
SCF, Human (P. pastoris) The protein was Expressed with an n-terminal Met. SCF (Glu26-Ala189) Accession # P21583-1. Biological activity: ED50 was < 2.0 ng/ mL by dose-dependent tF-1 cell stimulation, corresponding specific activity was 5 × 105IU /mg.
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