Reactive species: Human
Endotoxin: <2EU/ml
Concentration: 2 x 108Beads/mL
Application: T lymphocyte
Bioactivity: CD3/CD28 mab conjugated magnetic beads are mainly used for isolation, activation and in vitro amplification of human T cells. The use of 4.5-um superparamagnetic beads, matched to cell size, coupled with anti-CD3 and CD28 antibodies, provides the primary and co-stimulatory signals required for T cell activation and amplification. First, CD3/CD28 immunomagnetic beads were used for magnetic cell isolation, and CD3+ T cells were isolated and enriched from the obtained isolated products. After isolation, CD3+ T cells were cultured in the presence of magnetic beads. By binding anti-CD3 and anti-CD28 antibodies on immunomagnetic beads, the beads provide primary and costimulatory signals required for T cell activation and amplification. Activated T cells produce IL-2 (interleukin 2), GM-CSF (granulocyte macrophage stimulating factor), IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α), which play the role and function of T cells.
| Storage | 2~8℃ |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
Products are for research use only. Not for human use. We do not sell to patients.
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