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6-CFDA

Cat. No. M21717

All AbMole products are for research use only, cannot be used for human consumption.

6-CFDA Structure
Synonym:

6-Carboxyfluorescein diacetate

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Quality Control & Documentation
Biological Activity

6-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase.


Preparation of 6-CFDA working solution

1.Preparation of the stock solution
Dissolve 1mg 6-CFDA in 0.2172 mL DMSO to obtain 10 mM of 6-CFDA.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.

2.Preparation of 6-CFDA working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of 6-CFDA working solution.
Note: Please adjust the concentration of 6-CFDA working solution according to the actual situation.

Cell staining

1.Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.

2.Add 1 mL of 6-CFDA working solution, and then incubate at room temperature for 30 minutes.

3.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.

4.Wash twice with PBS, 5 minutes each time.

5.Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Precautions

1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.

2. Please adjust the concentration of 6-CFDA working solution according to the actual situation.

3. For your safety and health, please wear a lab coat and disposable gloves to operate.

Chemical Information
Molecular Weight 460.39
Formula C25H16O9
CAS Number 3348-03-6
Solubility (25°C) DMSO 90 mg/mL
Storage -20°C, protect from light
References

[1] Iigo Terrn, et al. Methods Enzymol. CFSE dilution to study human T and NK cell proliferation in vitro

[2] Mengting Jiang, et al. J Vis Exp. Shootward Movement of CFDA Tracer Loaded in the Bottom Sink Tissues of Arabidopsis

[3] Chih-Ju Lin, et al. Biomed Opt Express. Direct visualization of functional heterogeneity in hepatobiliary metabolism using 6-CFDA as model compound

[4] A Bruce Lyons, et al. Curr Protoc Cytom. Flow cytometric analysis of cell division by dilution of CFSE and related dyes

[5] Ben J C Quah, et al. Nat Protoc. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

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Keywords: 6-CFDA, 6-Carboxyfluorescein diacetate supplier, Fluorescent Dye, inhibitors, activators

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