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5-FAM (5-Carboxyfluorescein) is a green fluorescent reagent used for in situ labeling peptides, proteins and nucleotides. 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm. 5-FAM contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules.
| Molecular Weight | 376.32 |
| Formula | C21H12O7 |
| CAS Number | 76823-03-5 |
| Solubility (25°C) | DMSO 30 mg/mL |
| Storage | 4°C, protect from light |
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