Free shipping on all orders over $ 500
5-FAM SE is a green fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates. 5-FAM SE (5-Carboxyfluorescein succinimidyl ester) is chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP) isomers produced after reductive hydrolysis of pheomelanin with hydriodic acid.
| Molecular Weight | 473.39 |
| Formula | C25H15NO9 |
| CAS Number | 92557-80-7 |
| Solubility (25°C) | DMSO 20 mg/mL |
| Storage | -20°C, protect from light, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[3] H-J Lin, et al. Eye (Lond). The association between lumican gene polymorphisms and high myopia
| Related Fluorescent Dye Products |
|---|
| ANTS
ANTS is a fluorescent dye. ANTS and DPX are encapsulated in liposomes can be an effective approach for measuring membrane leakage. |
| Pafolacianine
Pafolacianine, also known as OTL0038 and OTL-38, is a folate receptor-directing imaging agent. Pafolacianine (OTL 38) is used in detecting ovarian cancer lesions. |
| Phalloidin-Tetramethylrhodamine
Phalloidin-retramethylrhodamine is an orange fluorescent probe (ex/em = 546/575 nm) that selectively binds to F-actin. It can be used to localize actin filaments in living or fixed cells, as well as for visualizing individual actin filaments in vitro. |
| 6-TAMRA
6-TAMRA has been a widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. The carboxylic acid of 6-TAMRA has also achieved prominence as a dye for oligonucleotide labeling and automated DNA sequencing applications. |
| 5-TAMRA
5-TAMRA is a pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.
