All AbMole products are for research use only, cannot be used for human consumption.
5-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase.
Preparation of 5-CFDA working solution
1.Preparation of the stock solution
Dissolve 1mg 5-CFDA in 0.2172 mL DMSO to obtain 10 mM of 5-CFDA.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
2.Preparation of 5-CFDA working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of 5-CFDA working solution.
Note: Please adjust the concentration of 5-CFDA working solution according to the actual situation.
Cell staining
1.Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.Add 1 mL of 5-CFDA working solution, and then incubate at room temperature for 30 minutes.
3.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
4.Wash twice with PBS, 5 minutes each time.
5.Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of 5-CFDA working solution according to the actual situation.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
| Molecular Weight | 460.39 |
| Formula | C25H16O9 |
| CAS Number | 79955-27-4 |
| Solubility (25°C) | DMSO ≥ 90 mg/mL |
| Storage | -20°C, protect from light |
| Related Fluorescent Dye Products |
|---|
| Coelenteramine 400a
Coelenteramine 400a (Coelenterazine 400a), a derivative of Coelenterazine, is a Renilla luciferase (RLuc) substrate. In the presence of Coelenteramine 400a, RLuc can emit blue light at 395 nm. |
| JC-10
JC-10 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential △Ψm. Can detect cell, tissue or purified mitochondrial membrane potential. When the mitochondrial membrane potential is high, JC-10 aggregates in the matrix of the mitochondria to form a polymer, which can produce red fluorescence; when the mitochondrial membrane potential is low, JC-10 cannot accumulate in the matrix of the mitochondria, at this time JC- 10 is a monomer, which can produce green fluorescence. In this way, it is very convenient to detect the change of mitochondrial membrane potential through the change of fluorescence color. |
| pH Fluorescent Probe Red 600, SE
pH Fluorescent Probe Red 600, SE is a pH-sensitive fluorescent dye, the fluorescence intensity changes significantly with changes in the pH of the environment. pH Fluorescent Probe Red 600, SE is weakly fluorescent outside the cells, but its fluorescence is significantly enhanced in acidic compartments (such as phagosomes, lysosomes and endosomes). It can be used for multiplexing cellular functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. Ex (nm) 576, Em (nm) 597 |
| DSPE-Rhodamine
DSPE-Rhodamine is formed by the conjugation of DSPE with the Rhodamine fluorescent dye. DSPE possesses a hydrophobic lipid tail and a hydrophilic head group, while Rhodamine is a red fluorescent dye. DSPE-Rhodamine retains the lipid properties of DSPE while also imparting fluorescent labelling capabilities. As a key component of delivery systems, DSPE-Rhodamine enhances targeting and bioavailability. Its lipid properties facilitate the penetration of molecules through cell membranes, while its fluorescent properties enable tracking of distribution and dynamic changes within the body. |
| LD540
LD540 is a novel high-sensitivity lipophilic dye modified with BODIPY fluorescent groups, designed for precise labelling and imaging of lipid droplets. LD540 exhibits excellent photostability and an optimal fluorescence spectrum, making it compatible with various commonly used fluorescent dyes (such as DAPI and Alexa Fluor 647), thereby supporting multi-colour imaging requirements. Additionally, LD540 is suitable for both fixed and live cells and can label ultra-small lipid droplets. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
