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5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.
| Molecular Weight | 473.39 |
| Formula | C25H15NO9 |
| CAS Number | 117548-22-8 |
| Solubility (25°C) | DMSO 50 mg/mL |
| Storage | -20°C, protect from light |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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| Phalloidin-Tetramethylrhodamine
Phalloidin-retramethylrhodamine is an orange fluorescent probe (ex/em = 546/575 nm) that selectively binds to F-actin. It can be used to localize actin filaments in living or fixed cells, as well as for visualizing individual actin filaments in vitro. |
| 6-TAMRA
6-TAMRA has been a widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. The carboxylic acid of 6-TAMRA has also achieved prominence as a dye for oligonucleotide labeling and automated DNA sequencing applications. |
| 5-TAMRA
5-TAMRA is a pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability. |
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