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5(6)-FAM SE

Cat. No. M21715
5(6)-FAM SE Structure

5(6)-Carboxyfluorescein succinimidyl ester mixed isomers

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10mg USD 60  USD60 In stock
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Biological Activity

5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

Chemical Information
Molecular Weight 473.39
Formula C25H15NO9
CAS Number 117548-22-8
Solubility (25°C) DMSO 50 mg/mL
Storage -20°C, protect from light
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Jagpreet S Nanda, et al. Methods Enzymol. Labeling a protein with fluorophores using NHS ester derivitization

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