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4-Thiouracil is a site-specific, photoactivatable probe used to detect RNA structures and nucleic acid-nucleic acid contacts. It absorbs ultraviolet light >300 nm and, in the presence of oxygen, acts as an energy donor to produce singlet oxygen by triplet-triplet energy transfer. The highly reactive oxygen species then reacts readily with 4-thiouracil, leading to the production of uracil and uracil-6-sulfonate, which is fluorescent at a wavelength of ~390 nm. 4-Thiouracil is suitable reagent employed in Schneider′s media for the embryos during RNA extraction. It may be employed as reagent for the Northwestern blotting technique.
| Molecular Weight | 128.15 |
| Formula | C4H4N2OS |
| CAS Number | 591-28-6 |
| Form | Powder |
| Solubility (25°C) | 1 M NaOH: 50 mg/mL |
| Storage | Store at -20°C |
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