Free shipping on all orders over $ 500

Sorafenib

Cat. No. M3026
Sorafenib Structure
Synonym:

BAY 43-9006

Size Price Availability Quantity
100mg USD 60 In stock
1g USD 120 In stock
Bulk Inquiry?

Quality Control
Biological Activity

Sorafenib is a multikinase inhibitor of Raf-1, B-Raf and VEGFR-2 with IC50 of 6 nM, 22 nM and 90 nM, respectively.

Protocol
Cell Experiment
Cell lines PLC/PRF/5, Hep3B, HepG2, HuH7
Preparation method PLC/PRF/5, Hep3B, HepG2 and HuH7 Cells were seeded in 96-well plates (3,000 cells/well) and incubated overnight for attachment, and were then treated with indicated agents in 10% FBS supplemented medium for 72 hours. The medium was replaced with MTT (0.5 mg/ml) at 37°C for 2 hours. After removal of medium, the cells were lysed with 200 μl per well dimethyl sulfoxide (DMSO), and absorbance at 570 nm was measured and the values of 50% inhibition concentration (IC50) for each drug were determined.
Concentrations 0, 0.25, 0.5,1, 2 µM
Incubation time 72 hours
Animal Experiment
Animal models PLC/PRF/5 xenograft model in female athymic nude mice of 4–5 weeks of age
Formulation Dissolved in Cremophor EL/ethanol (50:50; Sigma Cremophor EL, 95% ethanol) at 4 × concentration.
Dosages 60 mg/kg
Administration Po, qd
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 464.82
Formula C21H16ClF3N4O3
CAS Number 284461-73-0
Purity >99%
Solubility DMSO 80 mg/mL
Storage at -20°C
Customer Product Validations & Biological Datas
Source Phytomedicine (2015). Figure 1. Sorafenib was purchased from AbMole (Shanghai, China)
Method Cell viability was determined by MTT assay
Cell Lines HCC HepG2
Concentrations 0~100 µM
Incubation Time 24, 48, 72 hours
Results PPI dose- and time-dependently inhibited the proliferation of HepG2 cells, with IC50 values of 1.047 ± 0.376µM within 24 h. Sorafenib was used as a positive control in HepG2 cells.
Rating
Product Citations
References

Sorafenib and DE605, a novel c-Met inhibitor, synergistically suppress hepatocellular carcinoma
Xiufeng Jiang, et,al. Oncotarget, 2015,6;14.

Related Raf Products
L-779450

L-779450 is a potent, ATP-competitive Raf kinase inhibitor (IC50 =10 nM) that displays > 7, > 30 and > 70-fold selectivity over p38α, GSK3β and Lck respectively.

PF-04880594

PF-04880594 is a RAF inhibitor for BRAF/BRAFV599E and c-RAF with IC50 of 0.19 nM/0.13 nM and 0.39 nM, >100-fold selectivity over CSNK1, JNK2/3 and p38.

LGX818

LGX818 is a highly potent RAF inhibitor with selective anti-proliferative and apoptotic activity in cells expressing B-RAF(V600E) with EC50 of 4 nM.

GW5074

GW5074 is a potent and selective c-Raf inhibitor with IC50 of 9 nM, no effect on the activities of JNK1/2/3, MEK1, MKK6/7, CDK1/2, c-Src, p38 MAP, VEGFR2 or c-Fms is noted.

AZ628

AZ628 is a potent inhibitor for wild-type CRAF and BRAF V600E with IC50 of 29 nM and 34 nM, respectively.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Sorafenib, BAY 43-9006 supplier, Raf, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.