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Western Blot

Buffer/Detection

Cat.No.  Name Information
M10368 Gelatin (from porcine skin) Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology.
M9805 Glycerol Glycerol is a clear, colourless, viscous, sweet-tasting liquid. Glycerol is used in sample preparation and gel formation for polyacrylamide gel electrophoresis.
M9093 DTT Dithiothreitol (DTT) is a redox reagent commonly used as a reducing agent for thiolated DNA. Dithiothreitol (DTT) is also used to reduce the disulfide bonds of proteins.
M54672 Bovine Serum Albumin (Fatty Acid & IgG Free) BSA is a highly purified, lyophilized albumin, suitable for Westerns, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. It is also perfectly suitable as a nutrient in cell culture media, with low interference and backgrounds and is ideal for most standard molecular biology experiments.
M49893 Ammonium persulfate substitute Ammonium persulfate substitute (APS substitute) is a product used as a substitute for ammonium persulfate in PAGE and SDS-PAGE gel preparations.
M22515 Avidin (from egg white) Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
M11501 Polyvinylpyrrolidone Polyvinylpyrrolidone (PVP, Polyvidone, Povidone) is an inert, water-soluble, non-toxic, temperature-resistant, ph-stable, biocompatible, biodegradable polymer that helps encapsulate and meet the needs of hydrophilic and lipopphilic drugs. Polyvinylpyrrolidone has been widely tested and used as an effective wound healing promoter.
M11469 Acrylamide Acrylamide is a neurotoxic monomer with a wide range of industrial uses. It is a precursor compound of polyacrylamide.
M11401 Sodium lauryl sulfate Sodium dodecyl sulfate (SDS) is a kind of anionic surfactant, which is the first reported proteasome agonist, and has the characteristics of low concentration promotion and high concentration inhibition.
M10361 Albumin, Fatty Acid Free This product is bovine serum albumin (Fatty Acid Free BSA) without Fatty Acid, its Fatty Acid content is very low (0.02%), easy to dissolve.
M6559 CAPS CAPS is a buffer widely used in Western blotting.




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