Free shipping on all orders over $ 500


Cat. No. M6260
ROC-325 Structure
Size Price Availability
10mg USD 250 4-7 Days
25mg USD 450 4-7 Days
50mg USD 750 4-7 Days
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

In vitro: Treatment with ROC-325 triggers all of the hallmark features of autophagy inhibition including the accumulation of autophagosomes with undegraded cargo, an increase in lysosomal membrane permeability, deacidification of lysosomes, and elevated LC3B, p62, and cathepsin D expression. In vitro treatment of a panel of human AML cell lines and normal human bone marrow progenitors demonstrate that ROC-325 diminishes AML cell viability (IC50 range 0.7-2.2 µM), antagonizes clonogenic survival, and induces apoptosis in a manner that is therapeutically selective.

In vivo: ROC-325 is well tolerated and no notable toxicities are observed other than a modest, non-significant reversible reduction in mean body weight. Oral administration of ROC-325 to mice bearing RCC xenografts is well tolerated and yields dose-dependent inhibition of tumor growth that is significantly more efficacious than a higher dose of HCQ.

Cell Experiment
Cell lines RCC cells
Preparation method RCC cells were treated with ROC-325 for 24 h and harvested for imaging. Briefly, sections were cut in an LKB Ultracut microtome (Leica), stained with uranyl acetate and lead citrate, and examined in a JEM 1230 transmission electron microscope.
Concentrations 1 μM
Incubation time 24 h
Animal Experiment
Animal models Mice
Formulation water
Dosages 25, 40, and 50 mg/kg
Administration p.o.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 503.06
Formula C28H27ClN4OS
CAS Number 1859141-26-6
Purity >98%
Solubility 4 mg/mL in DMSO
Storage at -20°C

Drain the lysosome: Development of the novel orally available autophagy inhibitor ROC-325.
Carew JS, et al. Autophagy. 2017 Apr 3;13(4):765-766. PMID: 28118053.

Disruption of Autophagic Degradation with ROC-325 Antagonizes Renal Cell Carcinoma Pathogenesis.
Carew JS, et al. Clin Cancer Res. 2017 Jun 1;23(11):2869-2879. PMID: 27881580.

Related Autophagy Products

Autophinib is a novel potent autophagy inhibitor, inhibiting autophagy induced by starvation or Rapamycin with IC50 values of 90 and 40 nM, targeting the lipid kinase VPS34 with IC50 value of 19 nM in vitro.

Hemin chloride

Hemin is an iron-containing porphyrinis potentially for the treatment of gastroparesis.


AS1842856 is a cell-permeable Foxo1 inhibitor that blocks the transcription activity of Foxo1 with IC50 of 33 nM.


TBHQ is an aromatic organic compound, as a selective inducer of Nrf2 in a preclinical model of ICH.


Lys05 (Lys01 trihydrochloride) is a dimeric chloroquine (CQ) that deacidifies the lysosome and causes impairment of lysosomal enzymes, exhibiting more than 10-fold higher autophagy inhibitory potency than hydroxychloroquine (HCQ) and CQ (LC3II/LC3I ratio = 15.

Abmole Inhibitor Catalog 2017

Keywords: ROC-325 supplier, Autophagy, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.