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Vinblastine sulfate ia a plant alkaloid that arrests the cell cycle in G2/M phase by blocking mitotic spindle formation. Vinblastine sulfate inhibits normal microtubule assembly and causes aberrant tubulin polymerization. Vinblastine sulfate blocks binding of tubulin to microtubule proteins.Vinblastine sulfate Triggers Raf-1 activation, phosphorylation of Bcl-2 family proteins and induction of p53 expression. Cancer chemotherapeutic agent. Vinblastine sulfate is a microtubule disrupter. Vinblastine sulfate induces apoptosis in cultured hepatocytes and human lymphoma cells.Vinblastine sulfate shown to inhibit autophagosome maturation.
| Cell Experiment | |
|---|---|
| Cell lines | Chinese hamster ovary (CHO) cells |
| Preparation method | Six-well treatment plates are set up that contained 5 ×104 cells/mL in each well, suspended in 3 mL culture medium, and these are treated with vinblastine for 3 h followed by 21 h growth. |
| Concentrations | 1% (v/v) (dissolved in DMSO) |
| Incubation time | 3h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
| Molecular Weight | 909.05 |
| Formula | C46H60N4O13S |
| CAS Number | 143-67-9 |
| Purity | >98% |
| Solubility | DMSO |
| Storage | at -20°C |
Vinblastine-induced phosphorylation of Bcl-2 and Bcl-XL is mediated by JNK and occurs in parallel with inactivation of the Raf-1/MEK/ERK cascade.
Fan M, et al. J Biol Chem. 2000 Sep 29;275(39):29980-5. PMID: 10913135.
Caspase-3 activation is not responsible for vinblastine-induced Bcl-2 phosphorylation and G2/M arrest in human small cell lung carcinoma Ms-1 cells.
Tashiro E, et al. Jpn J Cancer Res. 1998 Sep;89(9):940-6. PMID: 9818030.
Comparison of the effects of vinblastine, vincristine, vindesine, and vinepidine on microtubule dynamics and cell proliferation in vitro.
Jordan MA, et al. Cancer Res. 1985 Jun;45(6):2741-7. PMID: 3986806.
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