Vinblastine sulfate ia a plant alkaloid that arrests the cell cycle in G2/M phase by blocking mitotic spindle formation. Vinblastine sulfate inhibits normal microtubule assembly and causes aberrant tubulin polymerization. Vinblastine sulfate blocks binding of tubulin to microtubule proteins.Vinblastine sulfate Triggers Raf-1 activation, phosphorylation of Bcl-2 family proteins and induction of p53 expression. Cancer chemotherapeutic agent. Vinblastine sulfate is a microtubule disrupter. Vinblastine sulfate induces apoptosis in cultured hepatocytes and human lymphoma cells.Vinblastine sulfate shown to inhibit autophagosome maturation.
|Cell lines||Chinese hamster ovary (CHO) cells|
|Preparation method||Six-well treatment plates are set up that contained 5 ×104 cells/mL in each well, suspended in 3 mL culture medium, and these are treated with vinblastine for 3 h followed by 21 h growth.|
|Concentrations||1% (v/v) (dissolved in DMSO)|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
Vinblastine-induced phosphorylation of Bcl-2 and Bcl-XL is mediated by JNK and occurs in parallel with inactivation of the Raf-1/MEK/ERK cascade.
Fan M, et al. J Biol Chem. 2000 Sep 29;275(39):29980-5. PMID: 10913135.
Caspase-3 activation is not responsible for vinblastine-induced Bcl-2 phosphorylation and G2/M arrest in human small cell lung carcinoma Ms-1 cells.
Tashiro E, et al. Jpn J Cancer Res. 1998 Sep;89(9):940-6. PMID: 9818030.
Comparison of the effects of vinblastine, vincristine, vindesine, and vinepidine on microtubule dynamics and cell proliferation in vitro.
Jordan MA, et al. Cancer Res. 1985 Jun;45(6):2741-7. PMID: 3986806.
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