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Trichostatin A

Cat. No. M1753
Trichostatin A Structure
Synonym:

TSA

Size Price Availability Quantity
2mg USD 100 In stock
5mg USD 210 In stock
10mg USD 330 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Trichostatin A (TSA) is an antifungal antibiotic derived from Streptomyces that inhibits mammalian histone deacetylase (HDAC). TSA inhibits the eukaryotic cell cycle during the beginning of the growth stage. Trichostatin A (TSA) can be used to alter gene expression by interfering with the removal of acetyl groups from histones (histone deacetylases, HDAC) and therefore altering the ability of DNA transcription factors to access the DNA molecules inside chromatin. It is a member of a larger class of histone deacetylase inhibitors (HDIs or HDACIs) that have a broad spectrum of epigenetic activities. Trichostatin A has also been shown to inhibit both G1- and G2-phases of the mammalian cell cycle and has been tested for use as a potential anticancer agent.

Protocol
Cell Experiment
Cell lines MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 cell lines
Preparation method Cell Proliferation Assay.
Stock cultures of breast cancer cell lines MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 (American Type Culture Collection, Rockville, MD) were grown in DMEM containing 10% (v/v) FCS, 2 mM L-glutamine, 100 units/ml penicillin, and 100 mg/ml streptomycin at 37°C in 5% CO2 humidified atmosphere. Cells were counted in a hemocytometer after detachment using 0.25% (w/v) trypsin in Dulbecco’s PBS without Ca21 or Mg21 (DPBS; Sigma-Aldrich) containing 0.02% (w/v) EDTA. Viability was determined by trypan blue exclusion. For each cell line, cells were seeded in 96-well microtiter plates at optimal densities determined in prior experiments to ensure exponential growth for the duration of the assay. After a 24-h preincubation, growth medium was replaced with experimental medium containing TSA at final concentrations ranging from 10-12 M to 10-5 M in log dilutions and 0.1% (v/v) ethanol, or growth medium containing 0.1% (v/v) ethanol as a vehicle control. After 96 h incubation, cell proliferation was estimated using the sulforhodamine B colorimetric assay (11), and the results are expressed as the mean 6 SD for six replicates as a percentage of vehicle control (taken as 100%).
Concentrations 10-12 M to 10-5 M
Incubation time 96 h
Animal Experiment
Animal models Inbred virgin female (Ludwig/Wistar/ Olac) rats bearing tumors induced with NMU model
Formulation DMSO
Dosages 500 µg/kg TSA in50 µl DMSO twice weekly for 4 weeks
Administration s.c. injection
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 302.37
Formula C17H22N2O3
CAS Number 58880-19-6
Purity >99%
Solubility DMSO 23 mg/mL
Storage at -20°C
References

Trichostatin A selectively suppresses the cold-induced transcription of the ZmDREB1 gene in maize.
Hu et al. PLoS One. 2011;6(7):e22132. PMID: 21811564.

Trichostatin A attenuates airway inflammation in mouse asthma model.
Choi et al. Clin Exp Allergy. 2005 Jan;35(1):89-96. PMID: 15649272.

Trichostatin A induces differential cell cycle arrests but does not induce apoptosis in primary cultures of mitogen-stimulated rat hepatocytes.
Papeleu et al. J Hepatol. 2003 Sep;39(3):374-82. PMID: 12927923.

Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo.
Vigushin et al. Clin Cancer Res. 2001 Apr;7(4):971-6. PMID: 11309348.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Trichostatin A, TSA supplier, HDAC, inhibitors

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