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Paeoniflorin

Cat. No. M3941
Paeoniflorin Structure
Synonym:

PF

Size Price Availability Quantity
50mg USD 100 In stock
100mg USD 150 In stock
200mg USD 250 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Paeoniflorin could activate PI3K/Akt signaling pathway to protect the PC12 cell injury induced by Aβ25-35.Paeoniflorin most likely contributes to the therapy for liver disease by exerting anti-inflammatory effects on HHSECs through blocking IL-8 secretion via downregulation of ERK1/2 and Akt.PF alleviates psoriatic skin lesions by inhibiting inflammation, which provides new insights into the immunomodulatory effect of PF in psoriasis treatment.Paeoniflorin pretreatment protects mice against Con A-induced liver injury via inhibition of several inflammatory mediators and, at least in part, by suppressing CD4(+), CD8(+) and NKT cell infiltration in liver. The beneficial effect of paeoniflorin may be related to the downregulation of TLR4 expression and the inhibition of NF-κB activation.Paeoniflorin alleviates liver fibrosis by inhibiting HIF-1α expression partly through mTOR pathway and paeoniflorin may be a potential therapeutic agent for liver fibrosis.

Protocol
Cell Experiment
Cell lines PC12 cells
Preparation method Cell Viability Assessment
Cell viability was measured using a quantitative colori-metric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyl tetrazolium bromide (MTT) (Sigma-Aldrich, USA) as reported previously (Mosmann 1983). Briefly, cells were seeded into collagen I-coated 96-well plates at a density of 1*104 per well. At the completion of the 24-h treatment, 10 μL MTT solution (5 mg/mL) was added. Following incubation for 4 h at 37 ℃, 100 μL dimethyl sulfoxide (DMSO) was used to dissolve crystals. The absorbance was measured using a microplate reader (Bio-Rad, USA) and cell viability was expressed as a percentage of control value.
Concentrations 0, 25, 50, 100, 200 and 400 μM
Incubation time 24 h
Animal Experiment
Animal models Adult male Sprague-Dawley rats
Formulation PBS, pH 7.5, 0.1 M at a concentration of 200 μg/μL
Dosages 15 mg/kg and 30 mg/kg for 20 consecutive days
Administration i.p.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 480.46
Formula C23H28O11
CAS Number 23180-57-6
Purity >99%
Solubility Ethanol
Water
Storage at -20°C
References

Paeoniflorin diminishes ConA-induced IL-8 production in primary human hepatic sinusoidal endothelial cells in the involvement of ERK1/2 and Akt phosphorylation.
Gong WG, et al. Int J Biochem Cell Biol. 2015 Mar 3;62:93-100. PMID: 25748730.

Paeoniflorin inhibits skin lesions in imiquimod-induced psoriasis-like mice by downregulating inflammation.
Sun Y, et al. Int Immunopharmacol. 2015 Feb;24(2):392-9. PMID: 25576402.

Paeoniflorin protects against concanavalin A-induced hepatitis in mice.
Chen M, et al. Int Immunopharmacol. 2015 Jan;24(1):42-9. PMID: 25479726.

Paeoniflorin alleviates liver fibrosis by inhibiting HIF-1α through mTOR-dependent pathway.
Zhao Y, et al. Fitoterapia. 2014 Dec;99:318-27. PMID: 25454463.

Paeoniflorin, a natural neuroprotective agent, modulates multiple anti-apoptotic and pro-apoptotic pathways in differentiated PC12 cells.
Wang D, et al. Cell Mol Neurobiol. 2013 May;33(4):521-9. PMID: 23436209.

Paeoniflorin attenuates amyloid-beta peptide-induced neurotoxicity by ameliorating oxidative stress and regulating the NGF-mediated signaling in rats.
Lan Z, et al. Brain Res. 2013 Mar 1;1498:9-19. PMID: 23295189.

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Keywords: Paeoniflorin, PF supplier, Akt, inhibitors

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