NPPB is a inhibits calcium-sensitive chloride currents (10 μM). Putative GPR35 agonist.
Acta Med Okayama. 2022 Aug;76(4):415-421.
5-Nitro-2-(3-phenylpropylamino) Benzoic Acid Inhibits the Proliferation and Migration of Lens Epithelial Cells by Blocking CaMKII Signaling
NPPB purchased from AbMole
Int J Mol Med. 2021 Apr;47(4):59.
5‑Nitro‑2‑(3‑phenylpropylamino) benzoic acid induces apoptosis of human lens epithelial cells via reactive oxygen species and endoplasmic reticulum stress through the mitochondrial apoptosis pathway
NPPB purchased from AbMole
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Source | Int J Mol Med (2021). Figure 2. NPPB (Abmole Bioscience Inc., USA) |
| Method | Mitochondrial membrane potential analysis | |
| Cell Lines | HLECs | |
| Concentrations | 50 µM | |
| Incubation Time | 24 h | |
| Results | JC‑1 aggregated in the mitochondria in the control group, with a ratio of 0.15±0.027, while the NPPB‑treated group exhibited a significantly higher ratio (0.59±0.080) since the monomeric form of JC‑1 was present in the cytosol |
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Source | Int J Mol Med (2021). Figure 1. NPPB (Abmole Bioscience Inc., USA) |
| Method | Cell viability assay | |
| Cell Lines | HLECs | |
| Concentrations | 10, 50, 100 and 200 µM | |
| Incubation Time | 24 h | |
| Results | Fig. 1 shows that NPPB significantly inhibited the viability of HLECs in a dose‑dependent manner, with an IC50 value of 53.36±0.1% (P<0.01 vs. 0 µM). These results suggested that NPPB decreased cell survival rate and inhibited the viability of HLECs. A concentration of 50 µM NPPB was used in subsequent experiments. |
| Molecular Weight | 300.31 |
| Formula | C16H16N2O4 |
| CAS Number | 107254-86-4 |
| Solubility (25°C) | DMSO ≥ 25 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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