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HO-3867

Cat. No. M3648
HO-3867 Structure
Size Price Availability Quantity
5mg USD 145 In stock
10mg USD 225 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

HO-3867 is a selective STAT3 inhibitor. HO-3867 blocks the JAK/STAT3 pathway in human ovarian cancer cell lines. HO-3867/cisplatin combination treatment significantly inhibites cisplatin-resistant cell proliferation in a concentration-dependent manner. HO-3867 induces G(2)-M cell cycle arrest in A2780 cells. HO-3867 promotes apoptosis by caspase-8 and caspase-3 activation.

Protocol
Cell Experiment
Cell lines A2780 (A2780R), PA-1, SKOV3, OV4, and OVCAR3 cells; human ovarian surface epithelial cell
Preparation method Determining cell viability by a colorimetric assay using MTT. In the mitochondria of living cells, yellow MTT undergoes a reductive conversion to formazan, producing a purple color. Cells, grown to ~80% confluence in 75-mm flasks, are trypsinized, counted, seeded in 96-well plates with an average population of 7,000 cells/well, incubated overnight, and then treated with HO-3867 for 24 h. All experiments are done using 8 replicates and repeated at least three times.
Concentrations ~20 μM
Incubation time 24 hours
Animal Experiment
Animal models Mice bearing ovarian cancer A2780 tumor xenografts
Formulation
Dosages ~100 ppm
Administration p.o.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 464.55
Formula C28H30F2N2O2
CAS Number 1172133-28-6
Purity >99%
Solubility DMSO
Storage at -20°C
Customer Product Validations & Biological Datas
Source APMIS (2015). Figure 4. HO-3867 (Abmole Bioscience, Shanghai, China)
Method The cellular growth, colony formation, and invasion assay
Cell Lines 143B cells
Concentrations
Incubation Time 24 h
Results In 143B cells, the cellular growth, colony formation, and invasion enhanced by LIF were partially neutralized by HO-3867 (Fig. 4B–D, p = 0.04, 0.016 and 0.05, respectively).
Rating
Product Citations
References

HO-3867, a curcumin analog, sensitizes cisplatin-resistant ovarian carcinoma, leading to therapeutic synergy through STAT3 inhibition.
Selvendiran K, et al. Cancer Biol Ther. 2011 Nov 1;12(9):837-45. PMID: 21885917.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: HO-3867 supplier, STAT, inhibitors

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