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Dorsomorphin dihydrochloride

Cat. No. M2238
Dorsomorphin dihydrochloride Structure
Synonym:

BML-275 2HCl

Size Price Availability Quantity
10mg USD 80 In stock
50mg USD 300 In stock
100mg USD 490 In stock
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Quality Control
Biological Activity

Dorsomorphin dihydrochloride is a potent inhibitor of AMP-activated protein kinase (AMPK) (Ki = 109 nM) and bone morphogenic protein (BMP) signaling. Dorsomorphin dihydrochloride inhibits AMPK activation induced by AICAR and metformin. Dorsomorphin dihydrochloride also inhibits the bone morphogenetic protein type 1 receptors ACTR-I (ALK2), BMPR-IA (ALK3), and BMPR-IB (ALK6). Dorsomorphin dihydrochloride induces autophagy in cancer cell lines via a mechanism independent of AMPK inhibition.

Protocol
Cell Experiment
Cell lines Spermatogonial Cell
Preparation method Dorsomorphin was added to the medium at a final concentration of 0, 1, 2, 4, or 8 µM (in final 0.4% dimethyl sulfoxide). The cells were maintained at 28.5°C in an atmosphere of 3% carbon dioxide and the medium was replaced every 3–4 days.
Concentrations 0, 1, 2, 4, or 8 µM
Incubation time 3 weeks
Animal Experiment
Animal models C57BL/6 mice
Formulation DMSO
Dosages 10 mg/kg
Administration i.p.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 472.41
Formula C24H25N5O.2HCl
CAS Number 1219168-18-9
Purity >99%
Solubility Water 94 mg/mL
Storage at -20°C
References

Dorsomorphin promotes survival and germline competence of zebrafish spermatogonial stem cells in culture.
Wong TT, et al. PLoS One. 2013 Aug 1;8(8):e71332. PMID: 23936500.

Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism.
Yu PB, et al. Nat Chem Biol. 2008 Jan;4(1):33-41. PMID: 18026094.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Dorsomorphin dihydrochloride, BML-275 2HCl supplier, AMPK, inhibitors

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