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Daphnetin

Cat. No. M3306
Daphnetin Structure
Size Price Availability Quantity
25mg USD 90 In stock
50mg USD 160 In stock
1g USD 780 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

It is also a chelator and an antioxidant. In vitro, daphnetin causes a 50% inhibition (IC50) of 3H-hypoxanthine incorporation by Plasmodium falciparum at concentrations between 25 and 40 uM.

Protocol
Cell Experiment
Cell lines MCF-7 cells
Preparation method Using the microculture MTT assay to estimate the cytostatic effect of Daphnetin which tested on the MCF-7 tumor cells . The assay is based on the reduction of soluble tetrazolium salt by mitochondria of viable cells. The reduced product,which is an insoluble purple-colored formazan, is dissolved in dimethyl sulfoxide and measured spectrophotometrically (570 nm). The amount of formazan formed is proportional to the number of viable cells. Cells (3 × 103) are seeded in each of the 96 microplate wells in a 200 μL medium which contains the corresponding concentration of daphnetin. Testing daphnetin at five concentrations (12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM). 24-hour, 48-hour, and 72-hour exposure later , the percentage of proliferative inhibition of treated cells is estimated against the solvent-treated control cells (PI% = [(T/C) − 1] × 100). PI = proliferation inhibition; T = treated, C = control. Calculating the IC50 from the least square concentration-response regressions
Concentrations 12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM
Incubation time 24 hours, 48 hours, and 72 hours
Animal Experiment
Animal models Immature CD1 (Im) female mice
Formulation Corn oil
Dosages 35 mg/kg, 70 mg/kg, and 140 mg/kg
Administration Subcutaneously
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 178.14
Formula C9H6O4
CAS Number 486-35-1
Purity >99%
Solubility DMSO 30 mg/mL
Storage at -20°C
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  Catalog
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