It is also a chelator and an antioxidant. In vitro, daphnetin causes a 50% inhibition (IC50) of 3H-hypoxanthine incorporation by Plasmodium falciparum at concentrations between 25 and 40 uM.
|Cell lines||MCF-7 cells|
|Preparation method||Using the microculture MTT assay to estimate the cytostatic effect of Daphnetin which tested on the MCF-7 tumor cells . The assay is based on the reduction of soluble tetrazolium salt by mitochondria of viable cells. The reduced product,which is an insoluble purple-colored formazan, is dissolved in dimethyl sulfoxide and measured spectrophotometrically (570 nm). The amount of formazan formed is proportional to the number of viable cells. Cells (3 × 103) are seeded in each of the 96 microplate wells in a 200 μL medium which contains the corresponding concentration of daphnetin. Testing daphnetin at five concentrations (12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM). 24-hour, 48-hour, and 72-hour exposure later , the percentage of proliferative inhibition of treated cells is estimated against the solvent-treated control cells (PI% = [(T/C) − 1] × 100). PI = proliferation inhibition; T = treated, C = control. Calculating the IC50 from the least square concentration-response regressions|
|Concentrations||12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM|
|Incubation time||24 hours, 48 hours, and 72 hours|
|Animal models||Immature CD1 (Im) female mice|
|Dosages||35 mg/kg, 70 mg/kg, and 140 mg/kg|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||DMSO 30 mg/mL|
|Related DNA/RNA Synthesis Products|
Vidarabine is an antiviral drug by interfering with the synthesis of viral DNA, used to treat herpes simplex and varicella zoster viruses.
Uridine is a nucleoside, contains a uracil attached to a ribose ring via a β-N1-glycosidic bond.
Rifampin is a DNA-dependent RNA polymerase inhibitor, used to treat a number of bacterial infections.
Mitomycin C is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers.
Mercaptopurine is a widely used antileukemic agent and immunosuppressive drug that inhibits de novo purine synthesis through incorporation of thiopurine methyltransferase metabolites into DNA and RNA.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.