It is also a chelator and an antioxidant. In vitro, daphnetin causes a 50% inhibition (IC50) of 3H-hypoxanthine incorporation by Plasmodium falciparum at concentrations between 25 and 40 uM.
|Cell lines||MCF-7 cells|
|Preparation method||Using the microculture MTT assay to estimate the cytostatic effect of Daphnetin which tested on the MCF-7 tumor cells . The assay is based on the reduction of soluble tetrazolium salt by mitochondria of viable cells. The reduced product,which is an insoluble purple-colored formazan, is dissolved in dimethyl sulfoxide and measured spectrophotometrically (570 nm). The amount of formazan formed is proportional to the number of viable cells. Cells (3 × 103) are seeded in each of the 96 microplate wells in a 200 μL medium which contains the corresponding concentration of daphnetin. Testing daphnetin at five concentrations (12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM). 24-hour, 48-hour, and 72-hour exposure later , the percentage of proliferative inhibition of treated cells is estimated against the solvent-treated control cells (PI% = [(T/C) − 1] × 100). PI = proliferation inhibition; T = treated, C = control. Calculating the IC50 from the least square concentration-response regressions|
|Concentrations||12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM|
|Incubation time||24 hours, 48 hours, and 72 hours|
|Animal models||Immature CD1 (Im) female mice|
|Dosages||35 mg/kg, 70 mg/kg, and 140 mg/kg|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||DMSO 30 mg/mL|
|Related DNA/RNA Synthesis Products|
Chebulinic acid is a potent natural inhibitor of M. tuberculosis DNA gyrase, also can inhibit SMAD-3 phosphorylation, inhibit H+ K+-ATPase activity.
TAS-102 is an orally administered combination of a thymidine-based nucleic acid analogue, trifluridine, and a thymidine phosphorylase inhibitor, tipiracil hydrochloride.
Brivudine is a uridine derivative and nucleoside analog with pro-apoptotic and chemosensitizing properties. It is incorporated into the viral DNA and blocks the action of DNA polymerases, thus inhibiting viral replication.
5-methoxyflavone is a novel DNA polymerase-beta inhibitor and neuroprotective agent against beta-amyloid toxicity.
5-BrdU is a synthetic thymidine analog; used for cell proliferation assays.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.